IFN-γ和IL-10作为药物抗结核活性评价指标的可行性考察

发布时间：2018-06-05 08:51

本文选题：结核 + 活菌计数　；参考：《中国药学杂志》2014年09期

【摘要】：目的探讨细胞因子是否可以作为一项指标评价药物对小鼠结核模型的疗效。方法 75只BALB/c小鼠雾化感染建立小鼠结核模型,分为13组,药物治疗2月以后,取血并做全肺活菌(CFU)计数;ELISA法检测血清中的γ-干扰素(IFN-γ)和白介素10(IL-10)的浓度;以全肺活菌计数作为药物对小鼠结核活性的标准,检验血中的γ-干扰素和白介素10的含量及二者的比值与小鼠肺中全肺活菌的相关性并进行线性回归。结果①全肺活菌计数:除左氧氟沙星(Lfx)和对氨基水杨酸(PAS)组以外,其余各治疗组与空白对照组相比均有统计学差异;各治疗组与阳性对照组(利福平+异烟肼+吡嗪酰胺,RHZ)相比均有统计学差异。②γ-干扰素:除左氧氟沙星、对氨基水杨酸组以外的各治疗组与空白对照组均有统计学差异;各治疗组与阳性对照组相比没有统计学差异。③白介素10:各治疗组与空白对照组、各治疗组之间均没有统计学差异。④γ-干扰素/白介素10:氯法齐明、利奈唑胺+丙硫异烟胺、利奈唑胺+氯法齐明、利奈唑胺+阿米卡星、利福平+异烟肼+吡嗪酰胺组与空白对照组相比有统计学差异,各治疗组之间均没有统计学差异。⑤γ-干扰素、白介素10、γ-干扰素/白介素10与全肺活菌呈正相关,确定因子r2分别为0.688、0.237、0.582。结论外周血中γ-干扰素的含量可以用来判断治疗方案是否有效,但不适宜作为生物标记物来预测体内结核菌的数量。
[Abstract]:Objective to investigate whether cytokines can be used as an index to evaluate the therapeutic effect of drugs on mice with tuberculosis. Methods Seventy-five BALB/c mice were induced by nebulization and were divided into 13 groups. After 2 months of drug therapy, the serum levels of IFN- 纬 and IL-10 were determined by Elisa. The live bacteria count of whole lung was used as the criteria for the activity of tuberculosis in mice. The contents of interferon 纬 and interleukin 10 in blood and the ratio of 纬-interferon and interleukin 10 in blood were determined and the correlation between them and the live bacteria in the whole lung of mice was analyzed and linear regression was carried out. Results 1the whole lung viable bacteria count: except levofloxacin Lfx) and p-aminosalicylic acid PAS-group, the other treatment groups were significantly different from the blank control group. There was statistical difference between each treatment group and the positive control group (rifampicin isoniazid pyrazinamide RHZ). 2. Interferon 纬: except levofloxacin, p-aminosalicylic acid group and blank control group; There was no statistical difference between each treatment group and the positive control group. There was no statistical difference between each treatment group and the blank control group. There was no statistical difference between the treatment group and the treatment group. There was a significant difference between the two groups, and there was no significant difference between the treatment group and the control group, and there was no significant difference between the treatment groups and the control group, and there was no significant difference in interferon 纬 between the treatment group and the control group, and there was no significant difference between the two groups. Interleukin-10, interferon- 纬 / interleukin-10 were positively correlated with live bacteria in the whole lung, and the determined factor R2 was 0.6880.237 and 0.582respectively. Conclusion the level of interferon 纬 in peripheral blood can be used to judge whether the therapeutic regimen is effective or not, but it is not suitable as a biomarker to predict the number of tuberculous bacteria in the body.
【作者单位】：首都医科大学附属北京胸科医院;北京市结核病胸部肿瘤研究所药物研究室;北京市结核病胸部肿瘤研究所药剂科;首都医科大学附属北京天坛医院药剂科学部;
【基金】：十二五“重大新药创新”科技重大专项(2012ZX09303002)
【分类号】：R521

【共引文献】