p38丝裂原活化蛋白激酶信号通路在阻滞剂HOE642对肺缺血再灌注保护中的作用

发布时间：2018-06-11 22:34

本文选题：缺血再灌注损伤 + 钠氢通道　；参考：《中南大学学报(医学版)》2017年07期

【摘要】：目的:探讨钠氢通道阻断剂HOE642对肺缺血再灌注损伤的保护作用,以及其保护作用与p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinase,p38MAPK)通路的关系。方法:选取野生小鼠36只,随机分为假手术组(SHAM组)、肺缺血再灌注组(I/R组)和肺缺血再灌注+HOE642组(HOE组),建立肺缺血再灌注损伤模型。测定肺组织含水量;光镜下观察肺组织病理学变化;ELISA检测炎症反应水平(IL-6,TNF-α);测定肺组织细胞内钙离子荧光强度;测定肺组织p38MAPK的蛋白表达。结果:HOE组肺组织含水量低于I/R组,但高于SHAM组(均P0.05);HOE组肺组织间质水肿、出血、炎症细胞浸润的情况比I/R组明显减轻,但比SHAM组严重(均P0.05);HOE组肺组织IL-6和TNF-α水平低于I/R组,但高于SHAM组(均P0.05);HOE组细胞内钙离子荧光强度低于I/R组,但高于SHAM组(均P0.05);HOE组肺组织p38MAPK的蛋白表达量低于I/R组,但高于SHAM组(均P0.05)。结论:HOE642可能通过调节p38MAPK信号通路,降低细胞内的钙离子浓度和钙超载,减轻炎症反应而发挥肺保护作用。
[Abstract]:Objective: To investigate the protective effect of sodium hydrogen channel blocker HOE642 on pulmonary ischemia reperfusion injury and the relationship between the protective effect and the p38 mitogen activated protein kinase (p38 mitogen activated protein kinase, p38MAPK) pathway. Methods: 36 wild mice were selected and randomly divided into the sham operation group (group SHAM), the lung ischemia reperfusion group (I/R group) and the lung. +HOE642 group (group HOE) of ischemia-reperfusion (group HOE), establish the model of lung ischemia reperfusion injury, determine the water content of lung tissue, observe the pathological changes of lung tissue under light microscope, ELISA test the level of inflammatory reaction (IL-6, TNF- a), determine the intracellular calcium fluorescence intensity of lung tissue, and determine the protein expression of p38MAPK in lung tissue. Results: the low water content of lung tissue in the HOE group is low. In group I/R, but higher than that in group SHAM (P0.05), the pulmonary interstitial edema, bleeding, and inflammatory cell infiltration in group HOE were significantly lower than those in the I/R group, but higher than those in the SHAM group (P0.05), and the levels of IL-6 and TNF- alpha in lung tissue of HOE group were lower than those in the I/R group, but higher than those in the SHAM group. The protein expression of p38MAPK in the lung tissue of HOE group was lower than that in group I/R, but higher than that in group SHAM (P0.05). Conclusion: HOE642 may reduce the intracellular calcium concentration and calcium overload and alleviate the inflammatory response by regulating the p38MAPK signaling pathway and exerting the protective effect of the lung.
【作者单位】：四川省医学科学院四川省人民医院麻醉科;四川大学华西医院麻醉科;
【基金】：国家自然科学基金(30571785) 四川省卫计委科研课题(16PJ458)~~
【分类号】：R563

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