枸杞多糖对COPD患者缺氧诱导因子1α抑制作用的研究

发布时间：2018-06-16 17:43

本文选题：COPD + LBP　；参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的明确枸杞多糖(LBP)对慢性阻塞性肺疾病(COPD)稳定期患者外周静脉血中缺氧诱导因子1α的抑制作用并探讨枸杞多糖浓度与HIF-1α的关系。方法1.收集101名入组对象,其中包括51例COPD稳定期患者,50例健康者,所有患者均有完整的临床资料。2.检测COPD稳定期组和健康对照组的血清HIF-1α指标,通过t检验进行组间比较,判断HIF-1α在COPD稳定期患者的表达水平。3.检测COPD稳定期组服用不同剂量枸杞多糖前、后血清HIF-1α指标,通过重复测量资料的方差分析,分析HIF-1α水平与剂量浓度及观察时间的关系。4.检测COPD患者动脉血气及肺功能指标。结果1.COPD稳定期患者外周静脉血中HIF-1α显著高于健康者,差异有统计学意义(P0.05)。2.COPD稳定期患者服用枸杞多糖2周后检测外周静脉血中HIF-1α表达水平较服用枸杞多糖前下降,差异有统计学意义(P0.05)。3.本实验中观察COPD稳定期患者服用不同剂量LBP后HIF-1α的变化趋势发现低剂量组下降趋势明显高于中、高剂量组。4.COPD稳定期组患者HIF-1α与PO2呈负相关(r=-0.49,P0.05),与PCO2呈正相关(r=0.283,P0.01);HIF-1α与患者的FEV1%呈负相关(r=-0.42,P0.05),与FEV1/FVC呈负相关(r=-0.54,P0.05)。5.服用枸杞多糖2周后,13例患者胸闷、气短症状改善,有效率30.23%;7例患者咳痰不利症状改善,有效率25.92%;7例患者体力增强、感冒次数减少,有效率17.95%;2例患者精神状态改善明显,有效率18.18%。结论1.COPD稳定期患者血清中HIF-1α表达水平较健康者明显升高。2.COPD稳定期患者血清中HIF-1α表达水平与机体缺氧、二氧化碳潴留及气流受限程度相关。3.LBP对COPD稳定期患者血清中HIF-1α表达具有抑制作用,本实验中低剂量LBP对HIF-1α的抑制作用优于中、高剂量组。4.LBP可以改善COPD患者的部分临床症状,提高其生活质量。
[Abstract]:Objective to investigate the inhibitory effect of Lycium barbarum polysaccharides (LBP) on hypoxia inducible factor-1 伪 (HIF-1 伪) in peripheral venous blood of patients with chronic obstructive pulmonary disease (COPD) at stable stage and to explore the relationship between LBPs concentration and HIF-1 伪. Method 1. 101 subjects were collected, including 51 stable COPD patients and 50 healthy subjects, all of whom had complete clinical data. 2. 2. Serum HIF-1 伪 levels were measured in stable COPD group and healthy control group. The expression level of HIF-1 伪 in stable COPD patients was determined by t test. Serum HIF-1 伪 was measured before and after different doses of Lycium barbarum polysaccharides in stable COPD group. The relationship between HIF-1 伪 level and dose concentration and observation time was analyzed by variance analysis of repeated measurement data. Arterial blood gas and pulmonary function were measured in COPD patients. Results 1. The level of HIF-1 伪 in peripheral venous blood of stable COPD patients was significantly higher than that of healthy controls. The difference was statistically significant (P 0.05). 2. The expression of HIF-1 伪 in peripheral venous blood of stable COPD patients was significantly lower than that before taking Lycium barbarum polysaccharides for 2 weeks. The difference was statistically significant (P 0.05). In this experiment, the trend of HIF-1 伪 in stable COPD patients with different doses of LBP was observed. The decrease trend of HIF-1 伪 in low dose group was significantly higher than that in middle dose group. 4. There was a negative correlation between HIF-1 伪 and PO2 in stable COPD group, a positive correlation between HIF-1 伪 and PCO2, a negative correlation between HIF-1 伪 and FEV1%, and a negative correlation between HIF-1 伪 and FEV1 / FVC, a negative correlation between HIF-1 伪 and FEV1 / FVC, a negative correlation between HIF-1 伪 and FEV1 / FVC, a negative correlation between HIF-1 伪 and FEV1 / FVC, a negative correlation between HIF-1 伪 and PCO2, a negative correlation between HIF-1 伪 and FEV1 / FVC, and a negative correlation with FEV1 / FVC, respectively. After taking Lycium barbarum polysaccharides for 2 weeks, 13 cases had chest tightness, the symptoms of shortness of breath were improved, the effective rate was 30.23%, the effective rate was 25.92%, the physical strength of 7 cases was enhanced, the number of colds was decreased, and the effective rate was 17.95%. The efficiency is 18.18. Conclusion 1. The expression of HIF-1 伪 in serum of stable COPD patients was significantly higher than that of healthy controls. 2. The expression of HIF-1 伪 in serum of stable COPD patients was significantly higher than that of healthy controls. The effect of low dose LBP on HIF-1 伪 expression in stable COPD patients was better than that in middle and low dose group. 4.LBP in high dose group could improve some clinical symptoms of COPD patients. Improve their quality of life.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R563.9

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