stim1和orai1蛋白在哮喘大鼠支气管平滑肌中的表达

发布时间：2018-06-18 04:14

本文选题：基质交感分子 + orai蛋白　；参考：《安徽医科大学学报》2014年04期

【摘要】：目的研究基质交感分子1(stim1)和钙离子释放激活钙离子通道蛋白1(orai1)在哮喘气道平滑肌细胞中表达水平的变化。方法选取40只清洁级SD大鼠随机分为对照组和哮喘组,其中哮喘组用鸡卵清蛋白(OVA)激发制备大鼠哮喘模型(n=20),对照组用生理盐水代替(n=20)。肺组织病理切片HE染色判断哮喘模型是否建立成功,分析支气管肺泡灌洗液(BALF)中的细胞总数及分类计数,应用免疫组化技术及彩色病理图文分析系统测定stim1和orai1蛋白阳性细胞平均光密度值。结果肺组织病理切片HE染色镜下观察呈现大量炎细胞浸润,支气管黏膜肿胀,支气管腔内可见黏液栓等,证实哮喘模型建立成功。哮喘组中大鼠BALF中细胞总数、中性粒细胞、嗜酸性粒细胞计数明显高于对照组,差异有统计学意义(P0.01)。哮喘组大鼠支气管平滑肌表达orai1蛋白阳性细胞平均光密度值明显高于对照组(P0.05)。哮喘组和对照组大鼠支气管平滑肌表达stim1蛋白阳性细胞的平均光密度值无明显差异(P0.05)。结论 orai1蛋白在哮喘大鼠气道平滑肌细胞中表达变化较stim1蛋白明显,对支气管平滑肌调节作用可能更重要。
[Abstract]:Objective to investigate the expression of matrix sympathetic molecule 1stim1 and Ca2 + releasing activated Ca2 + channel protein 1 in asthmatic airway smooth muscle cells (ASSMC). Methods Forty SD rats of clean grade were randomly divided into control group and asthmatic group. The asthmatic group was stimulated with ovalbumin (OVA) and the control group was treated with normal saline instead of OVA. Lung tissue sections were stained with HE to determine whether the asthma model was successful or not, and the total number of cells in bronchoalveolar lavage fluid (BALF) and its classification count were analyzed. The average optical density of stim1 and orai1 protein positive cells was measured by immunohistochemical technique and color pathological image and text analysis system. Results A large number of inflammatory cells, swelling of bronchial mucosa and mucus embolus in bronchial cavity were observed under HE staining in pathological sections of lung tissue. It was proved that asthma model was established successfully. The number of BALF cells, neutrophils and eosinophils in asthma group was significantly higher than that in control group (P 0.01). The average optical density of orai1 protein positive cells in bronchial smooth muscle in asthma group was significantly higher than that in control group (P 0.05). There was no significant difference in optical density between asthma group and control group in the expression of stim1 protein positive cells in bronchial smooth muscle. Conclusion the expression of orai1 protein in airway smooth muscle cells of asthmatic rats is more obvious than that of stim1 protein, which may play a more important role in regulating bronchial smooth muscle.
【作者单位】：安徽医科大学第一附属医院儿科;临沂市人民医院儿科;
【分类号】：R562.25

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