白介素17对急性肺损伤时肺水肿液清除的影响

发布时间：2018-06-18 13:54

本文选题：急性肺损伤 + 白介素　；参考：《南方医科大学学报》2017年04期

【摘要】：目的探讨白介素17(IL-17)对急性肺损伤(ALI)小鼠肺水清除的影响及可能机制。方法 IL-17基因敲除鼠和野生型C57BL/6鼠各16只,随机各等分为两组(一共4组,每组8只),分别经气道给与磷酸缓冲盐溶液和脂多糖(LPS)干预,给药48 h后处死。比较各组肺湿干质量比(W/D)、支气管肺泡灌洗液中白介素8(IL-8)水平和肺组织钠通道(ENa C)蛋白表达情况。并采用HE染色观察肺组织病理改变,免疫组化法了解肝激酶B1(LKB1)表达差异。结果与接受LPS干预的野生型鼠比较,IL-17敲除型鼠在接受LPS处理后W/D由9.739±3.3降至5.351±0.56,IL-8由67.50±7.33 pg/m L降至41.00±3.16 pg/m L,差异有统计学意义。病检结果提示IL-17敲除型鼠肺泡腔内水肿液聚集减少,肺损伤评分显著降低。Western blotting和免疫组化结果示接受LPS刺激的IL-17敲除型鼠ENa C表达更多,LKB1丢失相对较少。结论敲除IL-17不仅可以减轻ALI小鼠肺组织炎症程度,还可以减少ENa C蛋白丢失,促进肺水清除,其保护作用可能与LKB1有关。
[Abstract]:Objective to investigate the effect of interleukin 17 (IL 17) on the clearance of lung water in acute lung injury (Ali) mice and its possible mechanism. Methods 16 IL-17 knockout mice and 16 wild-type C57BL / 6 mice were randomly divided into two groups (4 groups, 8 rats in each group), treated with phosphate buffer solution and lipopolysaccharide (LPSs) respectively. The levels of interleukin-8 (IL-8) in bronchoalveolar lavage fluid (bronchoalveolar lavage fluid) and the protein expression of sodium channel (ENA) in lung tissue were compared. The pathological changes of lung tissue were observed by HE staining and the difference of expression of liver kinase B 1 and LKB 1 was detected by immunohistochemical method. Results compared with wild type mice treated with LPS, the WR / D decreased from 9.739 卤3.3 to 5.351 卤0.56 pg/m / L from 67.50 卤7.33 pg/m / L to 41.00 卤3.16 pg/m / L in LPS-treated mice. The results of pathological examination showed that the concentration of edema fluid in alveolar cavity of IL-17 knockout mice was decreased, the lung injury score was significantly decreased. Western blotting and immunohistochemical results showed that the expression of ENAC in LPS-stimulated IL-17 knockout mice was less than that in LPS-stimulated IL-17 knockout mice. Conclusion knockout of IL-17 can not only reduce the degree of lung inflammation in Ali mice, but also reduce the loss of ENAC protein and promote the clearance of lung water. The protective effect of IL-17 may be related to LKB1.
【作者单位】：重庆医科大学附属第二医院呼吸内科;重庆医科大学临床检验诊断学教育部重点实验室;
【基金】：国家自然科学基金(81670071)~~
【分类号】：R563

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