CKLF1-C19多肽对TGF-β诱导原代人肺成纤维细胞向肌成纤维细胞转化的干预作用

发布时间：2018-06-18 19:01

本文选题：CKLF-C多肽 + 肺成纤维细胞　；参考：《中国病理生理杂志》2017年11期

【摘要】：目的:探讨CKLF1-C19多肽(C19)对TGF-β诱导原代人肺成纤维细胞(LFB)向肌成纤维细胞(MFB)转化的干预作用。方法:培养原代人肺成纤维细胞并鉴定。用适宜的TGF-β浓度处理LFB,制备LFB向MFB转化的细胞模型。随后将实验分为对照组、TGF-β(5μg/L)组及4个浓度(1、0.1、0.01、0.001 mg/L)C19与TGF-β合用组。以细胞生长状态、细胞增殖率、α-平滑肌肌动蛋白(α-SMA)及I型胶原蛋白(collagen I)的表达变化为观察指标。结果:经显微镜检测,证实成功培养出原代人LFB。5μg/L TGF-β明显刺激LFB增殖及α-SMA和collagen I表达(P0.05)。与TGF-β组相比,C19-0.01 mg/L+TGF-β及C19-0.001 mg/L+TGF-β组LFB的细胞增殖率、α-SMA和collagen I的mRNA及α-SMA的蛋白表达均明显降低(P0.05)。结论:0.01 mg/L及0.001mg/L的CKF1-C19多肽对TGF-β诱导的LFB向MFB转化有一定的抑制作用,可在一定程度上抑制气道重塑和纤维化的发生。
[Abstract]:Aim: to investigate the effect of CKLF1-C19 polypeptide C19 on TGF- 尾 induced transformation of primary human lung fibroblast (LFB) to myofibroblast (MFB). Methods: primary human lung fibroblasts were cultured and identified. LFB was treated with appropriate TGF- 尾 concentration to prepare the cell model of LFB transforming into MFB. Then the experiment was divided into two groups: the control group (TGF- 尾 5 渭 g / L) and the combined group of 4 concentrations of TGF- 尾 0. 01 渭 g / L ~ (19) and TGF- 尾 0. 001 mg / L ~ (-1) ~ 0. 001 mg 路L ~ (-1) C ~ (19). The changes of cell growth state, cell proliferation rate, 伪 -SMAand collagen I expression were observed. Results: the primary human LFB.5 渭 g / L TGF- 尾 stimulated the proliferation of LFB and the expression of 伪 -SMA and collagen I by microscope. Compared with TGF- 尾 group, C19-0.01 mg / L TGF- 尾 and C19-0.001 mg / L TGF- 尾 decreased the cell proliferation rate, 伪 -SMA and collagen I mRNA and 伪 -SMA protein expression in LFB. Conclusion the CKF1-C19 peptide of 1: 0.01 mg / L and 0.001 mg / L can inhibit the transformation of LFB to MFB induced by TGF- 尾, and inhibit airway remodeling and fibrosis to some extent.
【作者单位】：广州医科大学附属第一医院广州呼吸疾病研究所国家呼吸疾病重点实验室;中山大学生命科学院;
【基金】：广州呼吸疾病国家重点实验室开放课题(No.SKLRD2010;No.SKLRD2014)
【分类号】：R56

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