茯苓、薏苡仁、冬瓜仁对肺纤维化大鼠肺组织TGF-β1、TNF-α影响的实验研究

发布时间：2018-06-18 21:48

本文选题：茯苓 + 薏苡仁　；参考：《青岛大学》2013年硕士论文

【摘要】：目的：借助大鼠肺纤维化动物模型,通过检测大鼠肺组织中TGF-β1、TNF-α的表达水平,探讨茯苓、薏苡仁、冬瓜仁治疗肺纤维化的疗效机制,为茯苓、薏苡仁、冬瓜仁的临床应用提供实验依据。方法：清洁级健康雄性Wistar大鼠,随机分为6组,每组12只,除正常对照组外,模型对照组、醋酸泼尼松组、茯苓组、薏苡仁组、冬瓜仁组以支气管内注射博莱霉素的方法制作肺纤维化动物模型,于造模后第2天给予相应药物干预；观察大鼠一般情况,给药后14天处死6只大鼠,28天处死余下大鼠,采集动物肺组织,光镜下观察大鼠肺组织病理变化情况,免疫组化法检测大鼠肺组织中TGF-β1、TNF-α的表达情况。采用SPSS17.0统计软件统计学处理相关实验数据。结果：实验第26天模型对照组死亡1只大鼠,各组大鼠存活率比较均无显著差异(P0.05)。博莱霉素干预的大鼠出现体重增长缓慢,甚至呼吸困难的症状。与正常对照组比较,模型对照组大鼠肺组织出现明显的肺纤维化,且TGF-β1、TNF-α表达显著高于正常对照组(P0.05),提示动物模型复制成功。与模型对照组比较,各治疗组明显改善肺纤维化模型大鼠肺组织的病理学改变,且TGF-β1、TNF-α水平均显著低于模型对照组(P0.05)；醋酸泼尼松组TGF-β1、TNF-α水平均低于其他各治疗组(P0.05)；茯苓组TGF-β1水平在14天、28天均低于薏苡仁组、冬瓜仁组(P0.05),薏苡仁组、冬瓜仁组之间比较无显著性差异；茯苓组TNF-α水平在28天低于薏苡仁组、冬瓜仁组(P0.05),TNF-α水平在14天三组之间无显著性差异。综上所述,三个中药组均可改善指标,茯苓组优于薏苡仁组、冬瓜仁组,但均不及醋酸泼尼松组改善程度显著。结论：茯苓、薏苡仁、冬瓜仁均能有效缓解肺纤维化大鼠的症状表现,抑制肺组织中TGF-β1及TNF-α的表达,推测三药可通过抑制TGF-β1及TNF-α的表达来延缓博莱霉素诱发的肺纤维化进程。
[Abstract]:Objective: To explore the therapeutic mechanism of Poria, coix seed and winter melon kernel in the treatment of pulmonary fibrosis by using rat model of pulmonary fibrosis and the expression level of TGF- beta 1 and TNF- alpha in rat lung tissue, and provide experimental basis for the clinical application of Poria cocos, coix seed and winter melon kernel.
Methods: healthy male Wistar rats were randomly divided into 6 groups, with 12 rats in each group. In addition to the normal control group, the model control group, the prednisone acetate group, the Poria group, the Coix seed group, the winter melon kernel group were injected with bleomycin in the bronchus to make the pulmonary fibrosis animal model, and the corresponding drug intervention was given second days after the model. 14 days after administration, 6 rats were killed and the remaining rats were killed for 28 days. The lung tissue was collected. The pathological changes of lung tissue were observed under light microscope. The expression of TGF- beta 1 and TNF- a in the lung tissue of rats was detected by immunohistochemical method. The data of the related experimental data were used by the statistical software of SPSS17.0.
Results: 1 rats were killed in the twenty-sixth day model control group. There was no significant difference in the survival rate of the rats in each group (P0.05). The rats with bleomycin intervened the slow weight growth and even the symptoms of dyspnea. Compared with the normal control group, the lung tissue of the model control group had obvious pulmonary fibrosis, and the expression of TGF- beta 1 and TNF- a was significant. Compared with the model control group, the pathological changes of lung tissue in the pulmonary fibrosis model rats were obviously improved, and the level of TGF- beta 1 and TNF- a was significantly lower than that of the model control group (P0.05), and the TGF- beta 1 and TNF- alpha water of the prednisone acetate group were lower than those of the other treatment groups (P0.05). The level of TGF- beta 1 in Poria group was lower than coix seed group in 14 days and 28 days, but there was no significant difference between winter melon kernel group (P0.05), coix seed group and winter melon kernel group. The level of TNF- alpha in Poria group was lower than coix seed group, winter melon kernel group (P0.05), and TNF- alpha level between the three groups in 14 days. All of the three Chinese medicine groups could improve. The index, Fuling group was better than that of Coix seed group, semen Benincasae group, but were less than the prednisone group improved significantly.
Conclusion: tuckahoe, coix seed and winter melon kernel can effectively alleviate the symptoms of pulmonary fibrosis in rats and inhibit the expression of TGF- beta 1 and TNF- alpha in lung tissue. Three drugs can delay the progression of bleomycin induced pulmonary fibrosis by inhibiting the expression of TGF- beta 1 and TNF- alpha.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R285.5;R563

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