慢性阻塞性肺疾病患者肺组织和血清HMGB1的表达及与TNF-α和IL-1β的相关性

发布时间：2018-06-19 19:07

本文选题：慢性阻塞性肺疾病 + 高迁移率蛋白　；参考：《中国老年学杂志》2014年18期

【摘要】：目的探讨慢性阻塞性肺疾病(COPD)患者肺组织和血清高迁移率族蛋白1(HMGB1)的表达及与肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β的相关性。方法采用RT-PCR及免疫组化染色法分别检测24例COPD伴肺癌患者(观察组)和24例无COPD肺癌患者(对照组)的肺组织中HMGB1的mRNA和蛋白表达,并采用ELISA法检测同期入院的COPD急性加重患者156例、稳定期患者80例和健康对照80例血清HMGB1、TNF-α和IL-1β水平,对结果进行比较并进行相关性分析。结果观察组24例标本中22例阳性,阳性率87.5%;对照组24例标本中10例染色阳性,阳性率41.6%(χ2=11.02,P0.01)。对照组HMGB1蛋白的平均光密度值显著低于观察组(t=7.91,P0.05)。对照组HMGB1的mRNA转录水平显著低于观察组(t=14.68,P0.01)。COPD急性期和COPD稳定期患者血清HMGB1、TNF-α、IL-1β水平相比对照组明显较高,且COPD急性期患者相比稳定期患者明显较高。重度COPD、肺部多发肺大泡患者血清HMGB1、TNF-α、IL-1β水平相比轻中度、肺部无肺大泡患者明显较高。无呼吸衰竭患者的HMGB1明显低于有呼吸衰竭患者,差异显著(t=2.122,P0.05),而呼吸是否衰竭在TNF-α、IL-1β水平上无差异。COPD急性加重期患者血清HMGB1与TNF-α、IL-1β水平存在显著相关(r=0.602、0.738,均P0.01)。结论 COPD病程中,HMGB1于肺组织和血清中高度表达,并同TNF-α和IL-1β等多种炎性介质相互作用。
[Abstract]:Objective to investigate the expression of high mobility group protein (HMGB1) in lung tissue and serum of patients with chronic obstructive pulmonary disease (COPD) and its correlation with tumor necrosis factor TNF- 伪 and interleukin (IL)-1 尾. Methods the expression of HMGB1 mRNA and protein in lung tissues of 24 COPD patients with lung cancer (observation group) and 24 patients without COPD lung cancer (control group) were detected by RT-PCR and immunohistochemical staining, respectively. The serum levels of HMGB1TNF- 伪 and IL-1 尾 were measured by Elisa in 156 patients with acute exacerbation of COPD, 80 patients with stable COPD and 80 healthy controls. The results were compared and analyzed. Results the positive rate was 87.5% in the observation group (22 cases) and 41.6% (10 / 24) in the control group (蠂 2 + 11.02% P 0.01). The average optical density of HMGB1 protein in the control group was significantly lower than that in the observation group. The mRNA transcription level of HMGB1 in the control group was significantly lower than that in the control group (P 0.01). The serum HMGB1 TNF- 伪 IL-1 尾 levels in the patients with COPD were significantly higher than those in the control group, and the levels of serum HMGB1 and TNF- 伪 IL-1 尾 in the COPD patients were significantly higher than those in the stable patients. The serum levels of HMGB1 TNF- 伪 and IL-1 尾 in patients with multiple pulmonary alveoli were significantly higher than those in patients without pulmonary alveoli. HMGB1 in patients without respiratory failure was significantly lower than that in patients with respiratory failure (P 0.05), but there was no difference in the level of TNF- 伪 and IL-1 尾 in patients with respiratory failure. There was a significant correlation between serum HMGB1 and TNF- 伪 and IL-1 尾 levels in patients with acute exacerbation of COPD, with a significant correlation between HMGB1 and TNF- 伪 -IL-1 尾 levels (P 0.01). Conclusion HMGB1 is highly expressed in lung tissue and serum during the course of COPD and interacts with many inflammatory mediators such as TNF- 伪 and IL-1 尾.
【作者单位】：宁夏医科大学总医院呼吸内科;
【基金】：宁夏自然科学基金(No.NZ13165)
【分类号】：R563.9

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