TRPC6在哮喘小鼠肺组织中的表达及其与气道炎症的相关性分析

发布时间：2018-06-23 07:08

本文选题：TRPC + NF-κB　；参考：《中国老年学杂志》2014年05期

【摘要】：目的探讨经典瞬时感受器电位通道(TRPC)6在哮喘小鼠肺组织中的表达情况及其与气道炎症的关系。方法采用卵白蛋白(OVA)致敏和激发建立哮喘模型,行支气管肺泡灌洗并对灌洗液进行细胞计数和分类,免疫组织化学染色观察肺组织TRPC6与细胞核转录因子(NF)-κB、细胞间黏附分子(ICAM)-1的表达。结果①哮喘组支气管肺泡灌洗液中白细胞(WBC)总数和嗜酸粒细胞百分比(EOS%)均明显增高,具有显著差异(P0.01);②免疫组化结果发现,哮喘小鼠肺组织TRPC6、NF-κB、ICAM-1蛋白的表达较正常组显著增加(P0.01),细胞分布定位具有一致性,主要在支气管上皮细胞和炎症细胞;③相关性分析显示,小鼠肺组织中TRPC6蛋白表达与NF-κB、ICAM-1表达及支气管肺泡灌洗液中WBC总数、EOS%呈显著性正相关(P0.05)。结论 TRPC6可能与哮喘小鼠肺组织气道炎症有关,从而参与哮喘的发病。
[Abstract]:Objective to investigate the expression of classical transient receptor potential channel (TRPC) 6 in lung tissues of asthmatic mice and its relationship with airway inflammation. Methods the asthmatic model was established by using ovalbumin (OVA) to sensitize and excite the asthmatic model. The bronchoalveolar lavage was performed and the cell count and classification of the lavage fluid were carried out. The expression of TRPC6, nuclear transcription factor (NF)-魏 B and intercellular adhesion molecule (ICAM)-1 in lung tissue was observed by immunohistochemical staining. Results (1) the total number of white blood cells (WBC) and the percentage of eosinophils (EOS%) in bronchoalveolar lavage fluid (BALF) in asthmatic group were significantly increased (P0.01). The expression of ICAM-1 protein in lung tissue of asthmatic mice was significantly higher than that of normal group (P0.01). The distribution and localization of ICAM-1 in lung tissue of asthmatic mice were consistent, mainly in bronchial epithelial cells and inflammatory cells. The expression of TRPC6 protein was positively correlated with the expression of NF- 魏 B, ICAM-1 and the total number of WBC in bronchoalveolar lavage fluid (EOS%) (P0.05). Conclusion TRPC6 may be involved in the pathogenesis of asthma in asthmatic mice.
【作者单位】：广州医科大学生理学教研室;
【基金】：广东省医学科研基金项目(A2013246) 广州市属高校科研计划项目(2012C043)
【分类号】：R562.25

【共引文献】