还原型谷胱甘肽对四氧化二氮致小鼠肺损伤的防护作用

发布时间：2018-06-26 23:38

本文选题：还原型谷胱甘肽 + 四氧化二氮　；参考：《食品工业科技》2016年18期

【摘要】：目的:探讨还原型谷胱甘肽(GSH)对四氧化二氮(N2O4)吸入致小鼠肺损伤的防护作用。方法:动物模型采取在120 L静式染毒柜内吸入N2O4的方法制作。雄性ICR小鼠64只,随机分为对照组,染毒组,GSH低、中、高剂量干预组(GSH低、中、高剂量+染毒),共五组,对照组8只,其余每组14只。染毒前,干预组分别灌胃GSH(1.25、2.50、3.75 g/kg bw·d),对照组和染毒组灌胃等体积生理盐水,连续7 d,第7 d灌胃1 h后染毒。脱臼处死动物,检测肺组织SOD、GSH-Px活性和MDA含量,观察肺病理改变。结果:与对照组比较,染毒组肺组织SOD、GSH-Px活性和MDA含量均极显著降低(p0.01);与染毒组比较,GSH低、中剂量干预组肺组织SOD和GSH-Px活性均极显著提高(p0.01),GSH低剂量干预组MDA含量极显著提高(p0.01),GSH中剂量干预组MDA含量显著提高(p0.05)。病理切片显示,GSH干预组比染毒组肺损伤程度轻。结论:GSH对N2O4吸入致小鼠肺损伤具有显著防护作用,其作用机制可能与其抑制氧化损伤有关。
[Abstract]:Aim: to investigate the protective effect of reduced glutathione (GSH) on lung injury induced by N _ 2O _ 4 inhalation in mice. Methods: the animal model was made by inhaling N _ 2O _ 4 in 120 L static poisoning cabinet. 64 male ICR mice were randomly divided into five groups: control group (n = 8), control group (n = 8) and control group (n = 14). Before administration, the intervention group was given GSH (1.25 ~ 2.50 ~ 3.75 g/kg bw d),) by intragastric administration for 7 days respectively, and the control group were given the same volume of normal saline for 1 h after 7 days. The dislocated animals were killed, the activity of GSH-Px and the content of MDA in the lung tissue were measured, and the pathological changes of the lung were observed. Results: compared with the control group, the activity of GSH-Px and the content of MDA in the lung tissue of the exposed group were significantly decreased (p0.01), and the GSH of the exposed group was lower than that of the exposed group. The activity of SOD and GSH-Px in lung tissue of middle dose intervention group was significantly increased (p0.01). The MDA content of GSH low dose intervention group was significantly increased (p0.01). The MDA content of middle dose GSH intervention group was significantly increased (p0.05). Pathological sections showed that the degree of lung injury in GSH treated group was lighter than that in exposed group. Conclusion GSH has a significant protective effect on lung injury induced by inhalation of N _ 2O _ 4 in mice, and its mechanism may be related to its inhibition of oxidative injury.
【作者单位】：军事经济学院军需系;军事经济学院医院;
【分类号】：R563

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