雾化吸入布地奈德干预肺纤维化大鼠及其机制的实验研究

发布时间：2018-06-30 01:27

  本文选题：肺纤维化 + 布地奈德　； 参考：《重庆医科大学学报》2014年09期

【摘要】：目的:探讨雾化吸入布地奈德对博莱霉素诱导的大鼠肺纤维化的影响及其可能机制。方法:45只SD雌性大鼠随机分为3组(各组15只):对照组、模型组和干预组,肺纤维化模型行气管内滴入博莱霉素造模。干预组造模后第7天雾化吸入布地奈德,模型组和对照组雾化吸入等量生理盐水,雾化后第7天、第14天、第28天各组随机处死5只动物并分别保存肺组织,HE和Masson染色判断肺组织肺泡炎及肺纤维化程度;免疫组化染色测定肺组织转化生长因子-β(transforming growth factor-β,TGF-β)、结缔组织生长因子(connective tissue growth factor,CTGF)的表达水平;RT-PCR检测Ⅰ、Ⅲ型胶原mRNA的表达水平。结果:肺组织病理学观察显示对照组肺泡炎和肺纤维化程度明显轻于模型组和干预组,干预组较模型组有明显改善;模型组和干预组肺组织中TGF-β和CTGF水平较对照组均有明显增高(P0.05),但干预组较模型组表达减少(P0.05);模型组Ⅰ、Ⅲ型胶原表达明显高于对照组(P0.05),而干预组较模型组表达明显减少(P0.05)。结论:布地奈德具有明显的抗纤维化作用,机制可能为通过抑制细胞因子的表达而减少肺组织中Ⅰ、Ⅲ型胶原的生成,最终抑制肺纤维化。
[Abstract]:Aim: to investigate the effect of inhaled budesonide on bleomycin induced pulmonary fibrosis in rats and its possible mechanism. Methods 45 female Sprague-Dawley rats were randomly divided into three groups (15 rats in each group): control group, model group and intervention group. Pulmonary fibrosis model was made by intratracheal instillation of bleomycin. In the intervention group, budesonide was inhaled on the 7th day after modeling, and the same amount of saline was inhaled by the model group and the control group, and on the 7th day and the 14th day after the atomization, budesonide was inhaled into the model group. On the 28th day, 5 animals were killed randomly and the lung tissues were preserved by HE and Masson staining to determine the degree of pulmonary alveolitis and pulmonary fibrosis. The expression of transforming growth factor- 尾 (TGF- 尾) and connective tissue growth factor (connective tissue growth factor- 尾 (CTGF- 尾) in lung tissue were detected by immunohistochemistry. Results: the pulmonary histopathological observation showed that the degree of alveolitis and pulmonary fibrosis in the control group was significantly less than that in the model group and the intervention group, and the intervention group was significantly improved compared with the model group. The levels of TGF- 尾 and CTGF in the model group and the intervention group were significantly higher than those in the control group (P0.05), but the expression of collagen 鈪，

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