骨髓间充质干细胞对染矽尘大鼠肺纤维化干预作用

发布时间：2018-07-03 00:28

本文选题：骨髓间充质干细胞 + 矽肺　；参考：《中国职业医学》2017年02期

【摘要】：目的探讨骨髓间充质干细胞(BMMSCs)对染矽尘大鼠不同时期肺纤维化的干预作用。方法无特定病原体级SD大鼠随机分为模型组、2周组、4周组和对照组,每组6只,雌雄各半。采用一次性非暴露式气管注入法,前3组大鼠予0.5 mL质量浓度为30 g/L的无菌矽尘悬液,对照组大鼠予等体积0.90%氯化钠溶液。2周组、4周组大鼠分别在染尘后第2和4周经尾静脉单次注射0.5 mL细胞密度为5×10~9/L的BMMSCs悬液,模型组和对照组大鼠予等体积0.90%氯化钠溶液。染尘后第12周对4组大鼠进行肺部计算机断层扫描(CT)检查,观察大鼠肺部组织病理改变,检测肺脏脏器系数、肺组织羟脯氨酸(HYP)及血清转化生长因子β1(TGF-β1)的水平。结果肺部CT显示:对照组大鼠肺野清透,肺纹理清晰;模型组大鼠肺部见弥漫分布、大小不一的颗粒状高密度影及条索状或网状纤维影;2周组大鼠肺部可见散在分布的细小颗粒状阴影;4周组大鼠肺部可见散在的颗粒状高密度影及网状阴影,颗粒大小及分布面积均小于模型组。模型组、2周组和4周组大鼠肺部CT值、肺脏脏器系数、肺组织HYP水平和血清TGF-β1水平均高于对照组(P0.05)。肺部CT值、肺组织HYP水平和血清TGF-β1水平从低到高依次为对照组2周组4周组模型组(P0.05);模型组和4周组大鼠肺脏脏器系数均高于2周组(P0.05)。结论 BMMSCs可减轻矽尘导致的肺纤维化,在纤维化早期应用效果更佳。
[Abstract]:Objective to investigate the effects of bone marrow mesenchymal stem cells (BMMSCs) on pulmonary fibrosis in rats exposed to silica. Methods Sprague-Dawley rats with no specific pathogens were randomly divided into two groups: model group (n = 6) and control group (n = 6). The rats in the first three groups were given aseptic silica dust suspension with 0.5 mL mass concentration of 30 g / L by a one-off non-exposed trachea injection, and the rats in the first three groups were given aseptic silica dust suspension. Rats in the control group were given BMMSCs with 0.5 mL cell density of 5 脳 10 9 / L at the 2nd and 4th week after exposure to 0.90% sodium chloride solution. The rats in the model group and the control group were given equal volume 0.90% sodium chloride solution. Lung computed tomography (CT) was performed in 4 groups of rats at the 12th week after dust exposure. The pathological changes of lung tissue, lung organ coefficient, the levels of hydroxyproline (HYP) and serum transforming growth factor 尾 1 (TGF- 尾 1) in lung tissue were observed. Results the lung CT showed that the lung field of the control group was clear and the lung texture was clear, and the diffuse distribution of the lung was found in the model group. Granular high-density shadow and stripe or reticular fiberoptic shadow were found in the lungs of the rats with different sizes, and the scattered fine granular shadows and reticular shadows could be seen in the lungs of the rats in the 4-week group, and the fine grained shadows and reticular shadows were found in the lungs of the rats in the 4-week group. The particle size and distribution area were smaller than the model group. The lung CT value, lung organ coefficient, HYP level and serum TGF- 尾 1 level in the model group were higher than those in the control group (P0.05). Lung CT value, HYP level and serum TGF- 尾 1 level from low to high were: control group, 4-week group model group (P0.05), model group and 4-week group lung organ coefficient were higher than 2-week group (P0.05). Conclusion BMMSCs can alleviate pulmonary fibrosis induced by silica dust, and the effect is better in early stage of fibrosis.
【作者单位】：贵州医科大学;广东省职业病防治院广东省职业病防治重点实验室;广东省妇幼保健院;
【基金】：国家科技支撑计划项目(2014BAI12B01) 国家自然科学基金(81302396) 国家临床重点专科建设项目(2011-09) 广东省医学科研基金(B2016056) 广东省职业病防治重点实验室(2012A061400007)
【分类号】：R135.2

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