鱼腥草提取物抗哮喘的实验研究

发布时间：2018-07-04 13:06

本文选题：鱼腥草 + 哮喘　；参考：《延边大学》2012年硕士论文

【摘要】：目的：研究鱼腥草(Houttuynia cor data Thunb)提取物雾化吸入抗哮喘的效果及有效浓度。方法：雄性BALB/C小鼠50只随机分成5组,每组10只。正常对照组(A组),模型组(B组),鱼腥草提物低剂量组(C组),鱼腥草提物中剂量组(D组),鱼腥草提物高剂量组(E组)。B组第1天和第7天给予50μg OVA和1mg氢氧化铝的生理盐水混合液200ul腹腔注射致敏,第14天、21天、22天、23天经呼吸道雾化吸入1.0%OVA (15ml)20min激发哮喘。C组、D组、E组分别在小鼠激发前1小时给予1mg/ml、10mg/ml、100mg/ml鱼腥草提物15ml雾化吸入20min。第24天处死小鼠后取BALF和末梢血,利用Diff-quik染色液检测嗜酸性粒细胞、中性粒细胞、单核细胞、淋巴细胞和巨噬细胞的数量；利用酶联免疫吸附法检测BALF离心上清液中的IL-4、IL-5、IL-6水平；利用H-E和刚果红染色观察肺部病理学改变情况。结果： 1.BALF检测结果：C组,B组与A组相比,BALF内嗜酸性粒细胞、淋巴细胞、中性粒细胞的数量显著增多；IL-4、IL-5、IL-6水平显著升高(p0.05)；D组、E组与B组相比,嗜酸性粒细胞、淋巴细胞、中性粒细胞数显著减少(p0.05)；IL-4、IL-5、IL-6水平显著降低(p0.05) 2.末梢血检测结果：B组与A组相比,嗜酸性粒细胞数量显著增多(p0.05)；C组,D组,E组分别与B组相比,嗜酸性粒细胞显著减少(p0.01)；D组,E组与C组相比,嗜酸性粒细胞明显减少(p0.01)；各实验组之间中性粒细胞、淋巴细胞、单核细胞无明显变化。 3.组织学观察显示鱼腥草提物中剂量组,高剂量组(E组)明显减轻气道及肺间质的病理学改变。结论：从实验结果可以得出以下结论,鱼腥草提物中剂量(10mg/ml),高剂量(100mg/ml)能有效的预防和抑制OVA诱导的小鼠哮喘发作。
[Abstract]:Objective: to study the effect and effective concentration of aerosol inhalation of Houttuynia cor data Thunb extract on asthma. Methods: fifty male BALB / C mice were randomly divided into 5 groups with 10 mice in each group. Normal control group (group A), model group (group B), low dose group (group C), middle dose group (group D) and high dose group (group E). Group B was given 50 渭 g of OVA and 1mg hydroxide on day 1 and 7. 200ul, a mixture of saline solution, was sensitized by intraperitoneal injection. Day 14, day 21, day 22, day 23, inhalation of 1.0 OVA (15ml) 20min to stimulate asthma. Group D and group E were given 1 mg / ml 10 mg / ml 15ml for 20 min, respectively. BALF and peripheral blood were taken from the mice on the 24th day. The numbers of eosinophils, neutrophils, monocytes, lymphocytes and macrophages were detected by Diff-quik staining solution, and the levels of IL-4 and IL-5- 6 in BALF centrifuge supernatant were detected by enzyme-linked immunosorbent assay (Elisa). The pathological changes of lung were observed by H-E and Congo red staining. Results: 1.The number of eosinophilic granulocytes, lymphocytes and neutrophils in BALF in group B was significantly increased compared with group A (p0.05) the eosinophilic granulocytes in group E were significantly higher than those in group B (p0.05). The number of lymphocytes and neutrophils decreased significantly (p0.05). The level of IL-4, IL-5 and IL-6 decreased significantly (p0.05). The results of peripheral blood test showed that the number of eosinophilic granulocytes in group B was significantly higher than that in group A (p0.05). The number of eosinophils in group D was significantly lower than that in group B, and the number of eosinophil in group E was significantly lower than that in group C. Eosinophilic granulocyte decreased significantly (p0.01), neutrophilic granulocyte, lymphocyte and monocyte did not change in each experimental group. Histopathological observation showed that the histopathological changes of airway and lung interstitium were significantly alleviated in middle dose group and high dose group (E group) of Houttuynia cordata extract. Conclusion: from the experimental results, the following conclusions can be drawn: middle dose (10mg/ml) and high dose (100mg/ml) of Houttuynia cordata extract can effectively prevent and inhibit OVA-induced asthma attack in mice.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R562.25

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