人脐带间充质干细胞对急性肺损伤氧化应激的影响

发布时间：2018-07-04 19:46

本文选题：人脐带间充质干细胞 + 肺损伤　；参考：《中国实用医药》2012年05期

【摘要】：目的探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUMSCs)对脂多糖(LPS)诱导的大鼠急性肺损伤(ALI)氧化应激失衡的作用。方法人脐带间充质干细胞的分离,培养和鉴定。将30只Wistar大鼠随机分为生理盐水(NS)对照组(A组)、LPS模型组(B组)和HUC-MSCs移植组(C组),每组10只。移植6h后,处死各组大鼠,取肺组织行病理学观察,肺湿/干重比测定,丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性检测。结果流式细胞术结果显示,纺锤样细胞高表达CD29、CD44和CD105,极低表达CD34,符合HUMSCs的特征。与A组比较,B组W/D和MDA含量明显升高(P0.05),SOD活性明显降低(P0.05)。与B组比较,C组W/D和MDA含量明显降低(P0.05),SOD活性明显升高(P0.05)。结论在大鼠模型中人脐带间充质干细胞移植能减轻肺损伤,并能降低肺组织MDA含量和提高SOD活性。
[Abstract]:Objective to investigate the effects of human umbilical cord mesenchymal stem cells (human umbilical cord mesenchymal stem cells) on oxidative stress imbalance induced by lipopolysaccharide (LPS) in rats with acute lung injury (Ali). Methods Human umbilical cord mesenchymal stem cells were isolated, cultured and identified. Thirty Wistar rats were randomly divided into normal saline (NS) control group (group A), LPS model group (group B) and HUC-MSCs transplantation group (group C) with 10 rats in each group. After 6 hours of transplantation, the rats in each group were killed. The lung tissues were taken for pathological observation, lung wet / dry weight ratio, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results the results of flow cytometry showed that the spindle like cells expressed CD29 CD44 and CD105, and the expression of CD34 was very low, which was consistent with the characteristics of HUMSCs. Compared with group A, WR D and MDA content in group B increased significantly (P0.05) and SOD activity decreased significantly (P0.05). Compared with group B, the content of WR D and MDA in group C decreased significantly (P0.05) and the activity of SOD increased significantly (P0.05). Conclusion Human umbilical cord mesenchymal stem cell transplantation can reduce lung injury, decrease MDA content and increase SOD activity in rat model.
【作者单位】：山西医科大学第二临床医学院呼吸内科;
【分类号】：R563.8

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