过氧化物酶体增殖因子活化受体γ在大鼠慢性阻塞性肺疾病模型中的表达及意义

发布时间：2018-07-05 16:51

本文选题：慢性阻塞性肺疾病 + 过氧化物酶体增殖因子活化受体γ　；参考：《中国老年学杂志》2017年10期

【摘要】：目的研究过氧化物酶体增殖因子活化受体(PPAR)γ在大鼠慢性阻塞性肺疾病(COPD)模型中的表达及意义。方法 SD大鼠24只,随机分为对照组(A组)和实验5 w组(B组)和实验10 w组(C组),每组8只。实验组采用气管内注入胰蛋白酶和熏香烟的方法,建立大鼠COPD模型。从开始实验起,实验第5周处死实验B组;第10周处死C组。应用病理学及肺功能检查评价COPD动物模型,免疫组化RT-PCR和Western印迹方法检测大鼠肺组织PPARγmRNA及蛋白的表达水平。结果 B组与A组比较,炎症反应明显,已形成慢性阻塞性肺气肿,并于第10周形成明显的肺气肿。PPARγ在正常肺组织与患慢性阻塞性肺气肿的肺组织均有表达,主要定位在炎性浸润细胞的核和核周围区,比较大鼠肺组织PPARγ在COPD中的表达(吸光度值A值):A组为0.497±0.078,B组PPARγ在肺组织中表达减弱(0.329±0.024),C组PPARγ在肺组织中表达明显减弱(0.216±0.049),差异均有统计学意义(P0.01)。PPARγmRNA表达随着COPD病程的进展逐渐降低,A组光密度比值为(1.06±0.10);B组为(0.50±0.02);C组为(0.27±0.02),与A组比较,B、C组差异有统计学意义(均P0.01)。Western印迹也表明COPD模型B组和C组比A组PPARγ蛋白表达降低,且B组蛋白表达降低的更为明显。结论 PPARγ在COPD的发生及进展中起重要作用,并且随着病程的发展,蛋白表达降低更为显著,因此该蛋白有望成为未来COPD治疗的新靶点。
[Abstract]:Objective to study the expression and significance of peroxisome proliferating factor-activated receptor (PPAR) 纬 in rat chronic obstructive pulmonary disease (COPD) model. Methods Twenty-four Sprague-Dawley rats were randomly divided into three groups: control group (group A), experimental group (group B) for 5 weeks and experimental group for 10 weeks (group C) with 8 rats in each group. Rat COPD model was established by intratracheal injection of trypsin and smoked cigarette. From the beginning of the experiment, group B was killed at the 5th week and group C was killed at the 10th week. The expression of PPAR 纬 mRNA and protein in rat lung tissue was detected by immunohistochemistry RT-PCR and Western blot. Results compared with group A, the inflammatory reaction in group B was obvious and the chronic obstructive emphysema had been formed, and the expression of PPAR 纬 in both normal lung tissues and lung tissues with chronic obstructive emphysema was observed at the 10th week. Mainly located in the nucleus and peri-nucleus of inflammatory infiltrating cells, The expression of PPAR 纬 in lung tissue of rats was significantly decreased (0.216 卤0.049) in group B (0.329 卤0.024) and group C (0.329 卤0.024). The difference was statistically significant (P0.01) .PPAR 纬 mRNA expression with the progression of COPD. The ratio of optical density in group A was (1.06 卤0.10) and (0.50 卤0.02) in group B was (0.27 卤0.02), which was significantly lower than that in group A (P0.01). Western blotting also showed that the expression of PPAR 纬 protein in group B and C was lower than that in group A. Moreover, the expression of B-histone decreased more obviously. Conclusion PPAR 纬 plays an important role in the pathogenesis and progression of COPD, and with the development of the course of disease, the expression of PPAR 纬 protein decreases more significantly. Therefore, PPAR 纬 may become a new target for the treatment of COPD in the future.
【作者单位】：首都医科大学附属北京世纪坛医院变态反应科;首都医科大学附属北京胸科医院重症监护室;北京大学医学部生物化学与分子生物学系;内蒙古电力中心医院包头医学院第二附属医院;
【分类号】：R-332;R563.9

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