壹期煤工尘肺患者血清氧化应激指标水平变化

发布时间：2018-07-10 08:30

本文选题：煤工尘肺(CWP) + 血红素氧合酶-(HO-)　；参考：《中国工业医学杂志》2014年06期

【摘要】：目的探讨壹期煤工尘肺(CWP)患者血清氧化应激指标血红素氧合酶-1(HO-1)、核因子E2相关因子2(Nrf2)、总抗氧化能力(T-AOC)、8-异前列腺素F2α(8-iso-PGF2α)及脂质过氧化产物(LPO)水平变化及意义。方法采用酶联免疫吸附法检测33名井上健康工人(对照组),40名井下健康接尘矿工(接尘组)及91例壹期CWP患者(CWP组)血清中HO-1、Nrf2和8-iso-PGF2α水平;化学比色法检测血清T-AOC和LPO水平。结果与对照组比较,接尘组Nrf2、T-AOC和LPO水平升高,CWP组LPO水平升高,差异均有统计学意义(P0.05,P0.01),CWP组T-AOC水平低于接尘组(P0.01),CWP组饮酒患者血清中HO-1、Nrf2和8-iso-PGF2α水平低于非饮酒者(P0.05)。CWP组HO-1和Nrf2、8-iso-PGF2α,Nrf2和8-iso-PGF2α水平呈明显正相关(rs值分别为0.840、0.882、0.885,P0.01)。结论氧化应激反应与壹期CWP发生发展有关;饮酒可影响CWP患者氧化应激水平;检测TAOC水平有助于CWP的早期筛检。
[Abstract]:Objective to investigate the changes and significance of serum oxidative stress indexes such as heme oxygenase-1 (HO-1), nuclear factor E2 related factor 2 (Nrf2), total antioxidant capacity (T-AOC), 8-iso-PGF2 伪 and lipid peroxidation products (LPO) in patients with coal worker pneumoconiosis (CWP). Methods the serum levels of HO-1Nrf2 and 8-iso-PGF2 伪 were measured by enzyme-linked immunosorbent assay (Elisa) in 33 healthy miners (control group) and 91 CWP patients (CWP group), and serum T-AOC and LPO levels were detected by chemical colorimetry. Results compared with the control group, the levels of Nrf2 T-AOC and LPO in the dust exposed group were higher than those in the CWP group, and the LPO level in the CWP group was higher than that in the control group. The levels of T-AOC in CWP group were significantly lower than those in CWP group (P0.01). The serum levels of HO-1nrf2 and 8-iso-PGF2 伪 in CWP group were significantly lower than those in non-drinkers (P0.05). The levels of HO-1, Nrf28-iso-PGF2 伪 Nrf2 and 8-iso-PGF2 伪 in CWP group were significantly higher than those in non-drinkers (rs = 0.8400.8820.885P0.01, respectively). Conclusion oxidative stress response is related to the development of CWP, alcohol consumption can affect the oxidative stress level of CWP patients, detection of TAOC level is helpful for early screening of CWP patients.
【作者单位】：开滦总医院检验科;河北联合大学;
【分类号】：R135.2

【参考文献】