慢性阻塞性肺疾病大鼠体内调节性T细胞、Th17细胞的变化

发布时间：2018-07-28 08:51

【摘要】：目的观察慢性阻塞性肺疾病(COPD)模型大鼠调节性T细胞(Treg)、Th17细胞的变化。方法将30只大鼠随机均分为正常组、模型组。模型组采用烟熏加脂多糖(LPS)气管滴入方法建立COPD肺气虚证大鼠模型,模型复制成功第28天,采用动物肺功能仪检测大鼠肺功能,酶联免疫吸附法检测大鼠血清白细胞介素(IL)-1β、IL-10、IL-17、IL-35水平,免疫组化法检测肺组织FoxP3、IL-17表达,流式细胞术检测外周血Treg表达。结果模型组大鼠肺泡腔及肺间质内大量炎性细胞浸润,肺组织结构破坏。与正常组比较,模型组大鼠肺功能参数0.3 s用力呼气容积(FEV0.3)、用力肺活量(FVC)、FEV0.3/FVC值降低(P0.01,P0.05);模型组大鼠血清IL-1β、IL-17、肺组织IL-17表达升高,血清IL-10、IL-35、肺组织FoxP3、外周血CD4+CD25+Treg表达降低(P0.01,P0.05);相关性分析显示,肺功能参数FEV0.3、FEV0.3/FVC、最大呼气流量(PEF)分别与IL-1β、IL-17呈负相关,与IL-35、FoxP3及CD4+CD25+Treg呈正相关(P0.05)。结论 COPD存在高炎症反应,CD4+CD25+Treg、Th17表达失衡,调节功能降低,导致炎症反应升高。
[Abstract]:Objective to observe the changes of regulatory T cell (Treg) Th17 cells in (COPD) rats with chronic obstructive pulmonary disease (COPD). Methods 30 rats were randomly divided into normal group and model group. In the model group, the rat model of COPD lung qi deficiency syndrome was established by fumigation and endotracheal instillation of lipopolysaccharide (LPS). The model was successfully replicated on the 28th day. The pulmonary function of the rats was measured by animal pulmonary function instrument. The level of IL-17 IL-35 in serum of rats was detected by Elisa. The expression of IL-17 in lung tissue was detected by immunohistochemistry, and the expression of Treg in peripheral blood was detected by flow cytometry. Results in the model group, a large number of inflammatory cells were infiltrated in the alveolar cavity and interstitium, and the lung tissue structure was destroyed. Compared with normal group, forced expiratory volume (FEV0.3), forced vital capacity (FVC) and FEV 0.3 / FVC were decreased in model group, serum IL-1 尾 -IL-17 and IL-17 expression in lung tissue were increased in model group. Serum IL-10 IL-35, lung tissue FoxP3, peripheral blood CD4 CD25 Treg expression decreased (P0.01P05), correlation analysis showed that the pulmonary function parameter FEV0.3 / FEV0.3 / FVCand maximal expiratory flow (PEF) were negatively correlated with IL-1 尾 -IL-17, and positively correlated with IL-35 FoxP3 and CD4 CD25 Treg (P0.05). Conclusion in COPD, the expression of CD4 CD25 Th17 is out of balance and the regulatory function is decreased, which leads to the increase of inflammatory response.
【作者单位】：湖北中医药大学研究生院;安徽中医药大学中西医结合临床学院;安徽医科大学第二附属医院中医科;安徽中医药大学第一附属医院呼吸科;
【基金】：国家自然科学基金(编号:81072781)
【分类号】：R563.9

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