RSV诱导免疫应答在COPD患者中的研究

发布时间：2018-07-31 17:24

【摘要】：目的:通过临床研究,探讨RSV能否诱导COPD患者发生免疫应答。方法:收集AECOPD组、SCOPD组患者及正常人血清/痰液标本各30例,用荧光定量PCR分别检测各组TLR3 mRNA、TLR4 mRNA,巢式PCR分别检测各组RSV,检出RSV者为阳性组,未检出RSV者为阴性组,并收集各组年龄、血气分析、血常规及肺功能,使用相关分析法分析TLR3 mRNA与肺功能的相关性,ELISA法检测IL-13、IL-32、INF-β、TNF-α及IRF-3的蛋白表达量。结果:1、AECOPD患者中检出RSV 8例,占26.6%,SCOPD患者中检出RSV 3例,占10%。2、RSV阳性组痰液/血清中IL-13、IL-32、INF-β、TNF-α及IRF-3的蛋白表达水平均较RSV阴性组高(p0.05)。3、RSV阳性组TLR3 mRNA、TLR4 mRNA水平较未检出组TLR3 mRNA、T LR4 mRNA水平高,且具有统计学意义(P0.05)。RSV阳性组中,痰液/血液中T LR3 mRNA表达增加与评价AECOPD严重程度的FEV1占预计值百分比呈负相关关系(r=-0.95)。结论:TLR3 mNRA表达增加与AECOPD患者肺功能下降密切相关,TLR3在介导抗病毒作用的同时,也产生炎症因子,能诱导免疫损伤,RSV阳性组的炎症因子较对照组明显增高,提示RSV是COPD的重要致病因子之一,RSV诱导的免疫反应可能参与COPD的免疫损伤。
[Abstract]:Objective: to investigate whether RSV can induce immune response in COPD patients. Methods: serum / sputum samples were collected from 30 patients with AECOPD and 30 normal controls. TLR3 mRNAs TLR4 mRNAs were detected by fluorescence quantitative PCR. Nested PCR was used to detect RSV in positive group and RSV negative group. Age, blood gas analysis, blood routine examination and lung function were collected. Correlation analysis was used to detect the protein expression of IL-13, IL-32, INF- 尾 -TNF- 伪 and IRF-3. Results 8 cases of RSV were detected in the patients with AECOPD, 3 cases of RSV were detected in 26.6 cases of SCOPD, and the protein expression levels of IL-13 mRNA-2INF- 尾 -TNF- 伪 and IRF-3 in sputum / serum of 10. 2RSV-positive patients were higher than those of RSV negative group (p0.05) .3The level of TLR3 mRNA-TLR4 mRNA was higher than that of undetected TLR3 mRNAT LR4 mRNA. There was a negative correlation between the increase of T LR3 mRNA expression in sputum / blood and the percentage of FEV1 in predicting the severity of AECOPD (r-0.95). Conclusion the increased expression of TLR3 mNRA is closely related to the decrease of lung function in patients with AECOPD. TLR3 not only mediates the antiviral effect, but also produces inflammatory factors. It is suggested that RSV is one of the important pathogenic factors of COPD. The immune response induced by RSV may be involved in the immune damage of COPD.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R563.9

【相似文献】