白藜芦醇对哮喘大鼠气道炎症及肺内诱导型一氧化氮合成酶的影响

发布时间：2018-08-05 17:17

【摘要】：目的观察白藜芦醇对哮喘大鼠气道炎症及肺内诱导型一氧化氮合成酶（iNOS）的影响。方法 40只SD大鼠随机分为4组：正常对照组（A组），哮喘模型组（B组），地塞米松干预组（C组）和白藜芦醇干预组（D组）（每组10只）。采用鸡清卵蛋白（OVA）和氢氧化铝（Al(OH)3）腹腔注射和雾化吸入激发的方法制备大鼠哮喘模型。硝酸还原法检测大鼠血清一氧化氮（NO）的水平；苏木精-伊红（HE）染色观察肺组织病理形态改变；免疫组织化学技术镜下观察肺组织核因子-κB（NF-κB）和iNOS的表达；免疫印迹（western blot）法测定肺组织中NF-κB蛋白表达水平。结果与A组比较，B组大鼠气道平滑肌增厚，炎性细胞浸润增多，血清NO含量的水平、肺组织NF-κB和iNOS的表达均增高，，差异有统计学意义（P0.05）；与B组比较，C组和D组气道平滑肌层较薄，炎症细胞浸润减轻；免疫组化显示，C组和D组中iNOS和NF-κB蛋白表达减弱，但均未达到正常水平，与B组比较有统计学意义（P 0.05）。结论白藜芦醇可减轻哮喘大鼠气道炎性细胞浸润及气管中血管和平滑肌增生，降低NF-κB和iNOS在肺组织中的表达及血清中NO的含量，表明白藜芦醇可通过抑制NF-κB和iNOS，降低血清NO含量，减少气道炎性细胞的募集，从而减轻气道炎症。
[Abstract]:Aim to observe the effects of resveratrol on airway inflammation and inducible nitric oxide synthase (iNOS) in lung of asthmatic rats. Methods Forty SD rats were randomly divided into 4 groups: normal control group (group A), asthma model group (group B), dexamethasone intervention group (group C) and resveratrol intervention group (group D). The asthmatic rat model was established by intraperitoneal injection of chicken ovalbumin (OVA) and aluminum hydroxide (Al (OH) 3 and inhalation of atomization. Nitric acid reduction assay was used to detect the level of nitric oxide (NO), hematoxylin-eosin (HE) staining was used to observe the pathological changes of lung tissue, and the expression of nuclear factor-魏 B (NF- 魏 B) and iNOS in lung tissue were observed by immunohistochemical technique. The expression of NF- 魏 B protein in lung tissue was detected by Western blot (western blot). Results compared with group A, the airway smooth muscle thickened, inflammatory cell infiltration increased, the level of serum no and the expression of NF- 魏 B and iNOS in lung tissue in group B were significantly higher than those in group A (P0.05). Compared with group B, the airway smooth muscle layer of group C and group D were thinner, and the infiltration of inflammatory cells decreased, and the expression of iNOS and NF- 魏 B in group C and group D decreased, but they did not reach the normal level, which was significantly higher than that in group B (P < 0. 05). Conclusion resveratrol can reduce the infiltration of airway inflammatory cells, vascular and smooth muscle hyperplasia in trachea, decrease the expression of NF- 魏 B and iNOS in lung tissue and the content of no in serum of asthmatic rats. Resveratrol can reduce airway inflammation by inhibiting NF- 魏 B and iNOS, reducing serum no content and reducing the recruitment of airway inflammatory cells.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R562.25

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