apelin-APJ在慢性阻塞性肺疾病大鼠肺组织中的表达及其意义
发布时间：2018-08-11 16:05
【摘要】：目的:检测慢性阻塞性肺疾病(COPD)大鼠肺组织中apelin及其受体APJ的mRNA和蛋白表达,探讨apelin-APJ系统在COPD发生、发展中的作用。方法:烟雾暴露法制造COPD大鼠模型。实验设对照组和COPD组。COPD组大鼠每日2次在熏烟箱内烟雾暴露,每次连续被动吸烟20支(1 h),持续4个月。qRT-PCR测定大鼠肺组织中apelin mRNA及APJ mRNA表达。免疫组化法检测大鼠肺组织apelin和APJ蛋白表达。结果:COPD组apelin、APJ mRNA相对表达量显著低于对照组(P0.01)。大鼠肺组织apelin、APJ mRNA表达与RV/(LV+S)均呈负相关(r=-0.454、-0.448,均P0.05);与FEV0.3/FVC呈正相关(r=0.529、0.475,均P0.05)。apelin、APJ蛋白主要表达在支气管肺组织上皮细胞、肺泡巨噬细胞、血管内皮细胞及部分肺泡壁细胞的细胞膜和细胞浆,COPD组及对照组apelin、APJ蛋白均呈阳性表达,半定量检测COPD组表达低于对照组(P0.05)。结论:apelin及其受体APJ的mRNA和蛋白在COPD大鼠肺组织表达均下降,可能是导致大鼠COPD肺动脉高压、肺心病发生发展的重要因素。apelin-APJ系统可能成为探索防治COPD肺动脉高压、右心室肥大的新靶点。
[Abstract]:Aim: to detect the expression of mRNA and protein of apelin and its receptor APJ in lung tissue of (COPD) rats with chronic obstructive pulmonary disease (COPD) and to explore the role of apelin-APJ system in the pathogenesis and development of COPD. Methods: COPD rat model was established by smoke exposure. The rats in the control group and the COPD group were exposed to smoke in the smoke box twice a day, and the expression of apelin mRNA and APJ mRNA in the lung tissue of the rats was determined by qRT-PCR, which lasted for 4 months (1 h), lasted 4 months). The expression of apelin and APJ in rat lung tissue was detected by immunohistochemical method. Results the relative expression of APJ mRNA in apelinia was significantly lower than that in control group (P0.01). The expression of APJ mRNA in rat lung tissue was negatively correlated with RV/ (LV S) (r = 0.4544-0.448, P 0.05), and positively correlated with FEV0.3/FVC (r = 0.529, P < 0.05, all P 0.05). The expression of APJ protein was mainly expressed in bronchopulmonary epithelial cells and alveolar macrophages. The expression of APJ protein in the plasma membrane and cytoplasm of vascular endothelial cells and some alveolar parietal cells was positive in COPD group and control group. The expression of APJ in COPD group was lower than that in control group (P0.05). Conclusion the decreased expression of mRNA and protein in lung tissue of rats with COPD may be an important factor in the pathogenesis and development of COPD pulmonary hypertension. The apelin-APJ system may be an important factor to explore the prevention and treatment of COPD pulmonary hypertension. A new target for right ventricular hypertrophy.
【作者单位】： 河北医科大学第一医院;邢台市人民医院;
【基金】：河北省卫生厅医学科学研究重点课题基金资助项目(编号:20090062)
【分类号】：R563.9
本文编号：2177489
[Abstract]:Aim: to detect the expression of mRNA and protein of apelin and its receptor APJ in lung tissue of (COPD) rats with chronic obstructive pulmonary disease (COPD) and to explore the role of apelin-APJ system in the pathogenesis and development of COPD. Methods: COPD rat model was established by smoke exposure. The rats in the control group and the COPD group were exposed to smoke in the smoke box twice a day, and the expression of apelin mRNA and APJ mRNA in the lung tissue of the rats was determined by qRT-PCR, which lasted for 4 months (1 h), lasted 4 months). The expression of apelin and APJ in rat lung tissue was detected by immunohistochemical method. Results the relative expression of APJ mRNA in apelinia was significantly lower than that in control group (P0.01). The expression of APJ mRNA in rat lung tissue was negatively correlated with RV/ (LV S) (r = 0.4544-0.448, P 0.05), and positively correlated with FEV0.3/FVC (r = 0.529, P < 0.05, all P 0.05). The expression of APJ protein was mainly expressed in bronchopulmonary epithelial cells and alveolar macrophages. The expression of APJ protein in the plasma membrane and cytoplasm of vascular endothelial cells and some alveolar parietal cells was positive in COPD group and control group. The expression of APJ in COPD group was lower than that in control group (P0.05). Conclusion the decreased expression of mRNA and protein in lung tissue of rats with COPD may be an important factor in the pathogenesis and development of COPD pulmonary hypertension. The apelin-APJ system may be an important factor to explore the prevention and treatment of COPD pulmonary hypertension. A new target for right ventricular hypertrophy.
【作者单位】： 河北医科大学第一医院;邢台市人民医院;
【基金】：河北省卫生厅医学科学研究重点课题基金资助项目(编号:20090062)
【分类号】：R563.9
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1 刘燕;兔急性肺动脉栓塞时Apelin-APJ系统的变化及意义[D];河北医科大学;2010年
，本文编号：2177489
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