贝母素甲对内毒素性急性肺损伤小鼠炎症因子表达的影响

发布时间：2018-08-13 12:11

【摘要】：目的探讨贝母素甲(PM)对脂多糖(LPS)诱导小鼠急性肺损伤过程中炎症因子表达的影响。方法 80只昆明种小鼠体重(20±5)g随机分成正常组、模型组、地塞米松(Dex)组、PM组,腹腔注射戊巴比妥(40 mg/kg)麻醉小鼠,模型组、Dex组及PM组小鼠给予气管内注射4 mg/kg LPS诱导建立急性肺损伤模型。注入LPS前1、12、24 h和36 h和注入后12 h,24 h和36 h,模型组、Dex组、PM组动物分别给予生理盐水、10 mg/kg盐酸地塞米松、1 mg/kg PM溶液,正常组给予同等体积生理盐水。实验结束后解剖肺脏,观察肺脏形态学改变,分别用Real-Time PCR和酶联免疫吸附试验(ELISA)测定肿瘤细胞坏死因子(TNF)-α、白介素(IL)-2、IL-6和IL-8。结果 LPS能明显引起小鼠肺部的炎症反应,PM处理后拮抗了LPS对小鼠肺组织的破坏作用,抑制促炎因子TNF-α、IL-2、IL-6、IL-8的表达。结论 PM对急性肺损伤小鼠肺组织保具有保护作用,其机制可能与下调炎症因子的表达有关。
[Abstract]:Objective to investigate the effect of (PM) on the expression of inflammatory factors during acute lung injury induced by lipopolysaccharide (LPS) in mice. Methods 80 Kunming mice with body weight (20 卤5) g were randomly divided into normal group, model group, dexamethasone (Dex) group and PM group. The mice were anesthetized by intraperitoneal injection of pentobarbital (40 mg/kg). Models of acute lung injury were induced by intratracheal injection of 4 mg/kg LPS in model group and PM group. The rats in the model group were given 10 mg/kg dexamethasone hydrochloride 1 mg/kg PM solution for 10 mg/kg in the model group and the same volume of normal saline in the normal group after injection of LPS for 24 h and 36 h and 12 h and 36 h after the injection, and the rats in the model group were given 10 mg/kg dexamethasone hydrochloride (Dexamethasone) 1 mg/kg PM solution, respectively. At the end of the experiment, the lung was dissected and the changes of lung morphology were observed. Tumor necrosis factor (TNF)-伪, interleukin (IL)-2-6 and IL-8 were measured by Real-Time PCR and enzyme linked immunosorbent assay (ELISA) respectively. Results LPS could obviously induce the inflammatory response in lung of mice treated with PM. It antagonized the destruction of lung tissue by LPS and inhibited the expression of IL-6 and IL-8 of TNF- 伪 and IL-2- 伪. Conclusion PM has protective effect on lung tissue protection in mice with acute lung injury, and its mechanism may be related to down-regulation of inflammatory factor expression.
【作者单位】：南阳医学高等专科学校基础医学部;
【基金】：河南省重点科技攻关项目(122102310103)
【分类号】：R563

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