槲皮素干预二氧化硅粉尘致肺纤维化大鼠的实验研究

发布时间：2018-08-16 15:54

【摘要】：目的观察槲皮素对二氧化硅粉尘所致肺纤维化的影响。方法选取48只雄性健康成年SPF级SD大鼠为研究对象,随机分为6组(对照组,槲皮素干预组,染尘7天、14天、21天和28天组)。对照组大鼠经气管注入无菌生理盐水1 ml,染尘组和槲皮素干预组大鼠注入50 mg/ml二氧化硅混悬液1 ml,预防组以50 mg/kg的槲皮素每天灌胃干预治疗,取肺组织进行HE染色,确定肺泡炎和肺纤维化程度,并检测肺组织中羟脯氨酸(HYP)含量以及过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果对照组大鼠肺组织结构基本正常;染尘组大鼠第7天时肺泡腔内可见大量炎性细胞浸润;14天时肺泡间隔内成纤维细胞显著增多,肺泡结构破坏;21天时大量巨噬细胞聚集形成细胞性结节且结节内有少量的胶原纤维;28天时肺纤维化程度进一步加重,形成广泛纤维化。随染毒时间的增加,肺组织中HYP的表达量逐渐升高,CAT及GSH-Px的活性逐渐降低;加入槲皮素后肺组织中HYP的表达量降低,CAT及GSH-Px的活性升高,差异具有显著性(P0.05)。结论槲皮素对矽尘所致的肺纤维化有一定的预防作用。
[Abstract]:Objective to observe the effect of quercetin on pulmonary fibrosis induced by silica dust. Methods Forty-eight male healthy adult SPF SD rats were randomly divided into 6 groups (control group, quercetin intervention group, dust exposure group for 21 days and 28 days group). Rats in the control group were injected with 1 ml of aseptic saline through trachea, the rats in the dust-infected group and quercetin intervention group were injected with 50 mg/ml silica suspension 1 ml. The prevention group was treated with 50 mg/kg quercetin daily and the lung tissues were stained with HE. To determine the degree of alveolitis and pulmonary fibrosis, and to detect the content of hydroxyproline (HYP) and the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in lung tissue. Results the lung tissue structure of the control group was basically normal and the number of fibroblasts in the alveolar septum was significantly increased after 14 days of infiltration of a large number of inflammatory cells in the alveolar cavity in the dust-exposed group. At 21 days after the destruction of alveolar structure, a large number of macrophages gathered to form cellular nodules and a small amount of collagen fibers were found in the nodules. At 28 days, the degree of pulmonary fibrosis was further aggravated and extensive fibrosis was formed. With the increase of exposure time, the expression of HYP in lung tissue increased gradually, the activities of cat and GSH-Px decreased gradually, and the expression of HYP in lung tissue decreased after adding quercetin, the activity of cat and GSH-Px increased significantly (P0.05). Conclusion Quercetin can prevent pulmonary fibrosis induced by silica dust.
【作者单位】：河北联合大学公共卫生学院/河北省煤矿卫生与安全实验室;河北联合大学医学实验动物中心;
【基金】：河北省应用基础研究项目(No.11966120D) 河北省科技支撑项目(No.02976194D)
【分类号】：R135.2

【相似文献】