接矽尘作业人员体内硅元素水平及与TGF-β1、TNF-α含量的关系

发布时间：2018-08-17 09:19

【摘要】：目的探讨接矽尘作业人员体内硅元素水平变化在矽肺发病机制、早期诊断中的意义。方法采用电感耦合等离子体质谱仪(ICP-MS)检测接矽尘作业工人、矽肺观察对象、矽肺患者及对照人群血清、尿液中硅元素含量,同时采用ELISA法检测其血清转化生长因子-β1(TGF-β1)及肿瘤坏死因子-α(TNF-α)含量。结果接矽尘组血清、尿液的硅元素水平明显高于对照组及矽肺组(P0.05);接矽尘组、观察对象组、矽肺组TGF-β1、TNF-α含量明显高于对照组(P0.05);接矽尘组接矽尘早期血清硅、尿硅含量即明显升高,随着接尘年限的增加,呈现降低的趋势;血清TGF-β1在接矽尘5年后明显升高,随着接尘年限的增长,呈现升高的趋势;血清TNF-α也呈现类似的变化趋势。结论接矽尘作业人员体内硅元素水平在接尘早期即明显升高且早于TGF-β1、TNF-α的改变,提示硅元素水平变化对矽肺发病机制研究及早期诊断具有重要意义。
[Abstract]:Objective to explore the significance of the changes of silicon level in silicosis exposed workers in the pathogenesis and early diagnosis of silicosis. Methods the contents of silicon in serum and urine of workers exposed to silica dust, silicosis patients, silicosis patients and controls were detected by inductively coupled plasma mass spectrometer (ICP-MS). The serum levels of transforming growth factor- 尾 1 (TGF- 尾 1) and tumor necrosis factor- 伪 (TNF- 伪) were determined by ELISA. Results the levels of silicon in serum and urine in silicosis group were significantly higher than those in control group and silicosis group (P0.05), and the TGF- 尾 1 TNF- 伪 content in silicosis group was significantly higher than that in control group (P0.05). Serum TGF- 尾 _ 1 increased significantly after exposure to silica dust for 5 years, and increased with the increase of exposure years, and serum TNF- 伪 showed a similar trend. Conclusion the level of silicon in workers exposed to silica dust increased significantly in the early stage of exposure to dust and was earlier than that of TGF- 尾 1 TNF- 伪, suggesting that the changes of the level of silicon in workers exposed to silica dust are of great significance in the study of pathogenesis and early diagnosis of silicosis.
【作者单位】：河北联合大学公共卫生学院;北京大学第三医院职业病研究中心;平顶山市职业病防治所;河北联合大学医学实验研究中心;
【基金】：河北省科学技术研究与发展计划项目(项目编号:092761100D)
【分类号】：R135.2

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