尼古丁对大鼠气道平滑肌细胞的促增殖作用

发布时间：2018-08-27 08:24

【摘要】：目的探讨尼古丁对大鼠气道平滑肌细胞增殖的作用。方法 (1)不同浓度的尼古丁作用大鼠气道平滑肌细胞24、48 h,应用细胞计数法检测大鼠气道平滑肌细胞的增殖变化;(2)不同浓度的尼古丁作用大鼠气道平滑肌细胞24 h,应用流式细胞术检测细胞周期变化;(3)1×10-5mol/L尼古丁分别作用大鼠气道平滑肌细胞12、16、20、24 h,Western blot检测Cyclin D1的表达水平。结果 (1)尼古丁组细胞计数均明显高于对照组,且尼古丁的促增殖作用与浓度和作用时间具有相关性。1×10-4、1×10-5、1×10-6、1×10-7mol/L尼古丁作用24 h,以对照组细胞计数值为1,则尼古丁组细胞计数分别为1.38、1.41、1.27、1.30,差异均有统计学意义(P0.05);尼古丁作用48 h,尼古丁组细胞计数分别为1.53、1.54、1.32、1.37,差异均有统计学意义(P0.05)。(2)各浓度尼古丁组S期的细胞分数均比对照组明显升高,差异有统计学意义(P0.05)。以对照组S期的细胞分数值为1,1×10-7、1×10-6、1×10-5、1×10-4mol/L尼古丁作用24 h,尼古丁组S期的细胞分数分别为1.53、2.35、3.83、2.86,差异均有统计学意义(P0.05)。(3)1×10-5mol/L尼古丁作用细胞12、16、20、24 h后,在12、20 h大鼠气道平滑肌细胞的Cyclin D1表达水平明显高于对照组,差异有统计学意义(P0.05)。结论尼古丁能够通过上调Cyclin D1促进大鼠气道平滑肌细胞的增殖。
[Abstract]:Objective to investigate the effect of nicotine on proliferation of rat airway smooth muscle cells. Methods (1) the proliferation of rat airway smooth muscle cells (ASMCs) was detected by cell count method at 24 h after exposure to different concentrations of nicotine, (2) the rat airway smooth muscle cells were treated with different concentrations of nicotine for 24 h. The changes of cell cycle were detected by flow cytometry. (3) 1 脳 10-5mol/L nicotine was used to detect the expression of Cyclin D1 in rat airway smooth muscle cells. Results (1) the cell count in nicotine group was significantly higher than that in control group. Moreover, the effect of nicotine on proliferation was correlated with the concentration and time of action. 1 脳 10-4, 1 脳 10-5, 1 脳 10-6, 1 脳 10-6, 1 脳 10-7mol/L for 24 h, and the cell count of control group was 1, the cell count in nicotine group was 1.38 1 1 1. 27 1. 30, the difference was statistically significant (P0.05); the effect of nicotine on nicotine was significant (P0.05). At 48 h, the cell counts in nicotine group were 1.53n 1.54U 1.32U 1.37.The difference was statistically significant (P0.05). (2). The cell fractions of S-phase in nicotine group were significantly higher than those in control group. The difference was statistically significant (P0.05). The cell fraction of S phase in control group was 1 脳 10 ~ (-7) C ~ (-1) 脳 10 ~ (-6) C ~ (-1) 脳 10 ~ (-5) N ~ (-1) 10-4mol/L nicotine for 24 h, and the cell fraction in S phase of nicotine group was 1.53C ~ (2.35) C ~ (3) 3.83 ~ (3) C ~ (-1) 2.86, respectively. The difference was significant (P0.05). (3) 1 脳 10-5mol/L nicotine treated cells for 24 h. The expression of Cyclin D1 in airway smooth muscle cells was significantly higher than that in the control group at 1220 h (P0.05). Conclusion nicotine can promote the proliferation of rat airway smooth muscle cells by up-regulating Cyclin D1.
【作者单位】：广州医科大学形态学实验中心;广州医科大学附属第一医院、呼吸疾病国家重点实验室;
【基金】：广州医科大学博士、留学回国人员科研项目(编号:2013C09)
【分类号】：R563.9

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