MiRNA-1247-3P在急性呼吸窘迫综合征细胞模型中的表达及功能分析

发布时间：2018-08-29 17:48

【摘要】：目的:检测脂多糖(LPS)处理A549细胞前后发生显著变化的mi RNAs,并验证mi R-1247-3P在不同浓度LPS处理后细胞中表达量的变化,并探讨其可能机制。方法:对照组是以培养液处理A549细胞,实验组分别以不同浓度的LPS处理A549细胞。通过免疫细胞化学染色法、RT-PCR方法检测各组细胞中的SP-A、SP-C的表达量。用基因芯片检测细胞处理前后的表达谱,找出其中的关键mi RNA,PCR的方法检测各组细胞中mi RNA的表达量。结果:实验组中各组细胞的SP-A、SP-C的表达量较对照组均下降(P0.05)。mi RNAs芯片检测结果显示:表达显著上调的有31个,显著下调的有3个。实时荧光定量PCR测出各实验组mi RNA-1247-3P的相对表达量较对照组均上升(P0.05)。结论:mi RNA-1247-3P在实验组有高表达,提示其可能在ARDS的发生发展过程中发挥作用。
[Abstract]:Aim: to detect the significantly changed mi RNAs, of A549 cells treated with lipopolysaccharide (LPS) and to verify the changes of mi R-1247-3P expression in A549 cells treated with different concentrations of LPS, and to explore the possible mechanism of mi R-1247-3P expression in A549 cells. Methods: A549 cells were treated with culture medium in control group and A549 cells were treated with different concentrations of LPS in experimental group. The expression of SP-A,SP-C was detected by immunocytochemical staining and RT-PCR. The gene chip was used to detect the expression of mi RNA in the cells before and after treatment, and the key mi RNA,PCR was found. Results: compared with the control group, the expression of SP-A,SP-C in the experimental group was lower than that in the control group (P0.05) .mi RNAs chip analysis showed that the expression of SP-A,SP-C was significantly up-regulated in 31 cells and significantly down-regulated in 3 cells. The relative expression of mi RNA-1247-3P in each experimental group was higher than that in the control group by real-time fluorescence quantitative PCR (P0.05). Conclusion the high expression of RNA-1247-3P in the experimental group suggests that it may play a role in the pathogenesis and development of ARDS.
【作者单位】：郑州大学人民医院儿科(河南省人民医院儿科);河南省开封市儿童医院;南方医科大学珠江医院儿科;广州市妇女儿童医疗中心;
【分类号】：R563.8

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