PPAR-γ激动剂吡咯列酮对卵蛋白激发气道变应性炎症的调节作用

发布时间：2018-08-29 19:37

【摘要】：目的探讨过氧化物酶增殖激活受体(PPAR)γ激动剂吡咯列酮对卵蛋白(OVA)激发哮喘小鼠气道变应性炎症的作用及机制。方法采用OVA激发制备小鼠气道变应性炎症反应模型,同时给予PPARγ激动剂吡格列酮(PGZ,10mg/kg)干预,采用有创肺功能检查、细胞分类计数、ELISA以及组织病理学检查等方法观察气道反应性、血清免疫球蛋白、肺泡灌洗液(BAL)细胞总数、分类计数以及细胞因子、化学因子和支气管肺组织病理学改变。结果OVA可诱导小鼠气道高反应性和嗜酸性粒细胞炎症,BAL中细胞总数增加,嗜酸粒细胞升高。血清总IgE、IgG1以及特异性IgE、IgG1、IgG2a增加(P0.01),但总IgG2a仅轻度升高。BAL中IL-4、IL-13、IL-17A和eotaxin、IFN-γ、MCP-1均明显增加(P0.01)。气道及肺血管周围炎症细胞浸润(P0.01)。PPAR-γ激动剂PGZ可部分抑制小鼠气道高反应性、BAL细胞总数及嗜酸粒细胞百分比增加,同时IL-4、IL-13、IL-17A、eotaxin和IFN-γ也被部分抑制(P0.05),MCP-1仅被轻度抑制(P0.05)。相应的病理学改变也被部分逆转(P0.05)。而血清总的IgE、IgG1、IgG2a以及特异性IgE、IgG1、IgG2a未见明显变化(P0.05)。结论 PPAR-γ激动剂PGZ对OVA激发小鼠气道变应性炎症反应具有调节作用。
[Abstract]:Objective to investigate the effect and mechanism of peroxidase proliferation-activated receptor (PPAR) 纬) agonist pyrrolidone on allergic airway inflammation induced by ovalbumin (OVA) in asthmatic mice. Methods the model of allergic airway inflammation in mice was induced by OVA. The airway reactivity was observed by PPAR 纬 agonist pioglitazone (PGZ,10mg/kg). The airway reactivity was observed by invasive pulmonary function test, cell classification count Elisa and histopathological examination. Serum immunoglobulin, total number of (BAL) cells in alveolar lavage fluid, classification count, cytokines, chemical factors and histopathological changes of bronchoalveolar lavage fluid. Results OVA could induce the increase of the total number of cells and the increase of eosinophils in airway hyperresponsiveness and eosinophil inflammation. Serum total IgE,IgG1 and specific IgE,IgG1,IgG2a were increased (P0.01), but IL-4,IL-13,IL-17A and eotaxin,IFN- 纬 -MCP-1 in total IgG2a increased slightly (P0.01). Airway and pulmonary perivascular inflammatory cell infiltration (P0.01). PPAR- 纬 agonist PGZ could partially inhibit the increase of the total number and percentage of eosinophilic granulocytes in mice airway hyperresponsiveness, while IL-4,IL-13,IL-17A,eotaxin and IFN- 纬 were also partially inhibited (P0.05) and MCP-1 was only slightly inhibited (P0.05). The corresponding pathological changes were also partially reversed (P0.05). There was no significant change in serum total IgE,IgG1,IgG2a and specific IgE,IgG1,IgG2a (P0.05). Conclusion PPAR- 纬 agonist PGZ can regulate allergic airway inflammation induced by OVA in mice.
【作者单位】：西安交通大学医学院第二附属医院呼吸内科;西安交通大学生命科学学院;
【基金】：国家自然科学基金资助项目(No.81000009) 中央高校基本科研业务费(No.18630006)~~
【分类号】：R562.25

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