急性肺损伤大鼠肺神经导向因子Slit2及Robo4表达的研究

发布时间：2018-09-01 08:55

【摘要】：目的：观察盲肠结扎穿孔致急性肺损伤(ALI)大鼠肺脏神经导向因子Slit2及Robo4的改变，探讨二者在ALI发生、发展中的作用。方法：72只SD雄性大鼠随机分为对照组(n=24)与ALI(n=48)组。ALI组采用盲肠结扎穿孔术复制ALI模型，对照组只开腹、关腹，不行盲肠结扎穿孔术。于致伤后12、24、48h取大鼠动脉血测定动脉血氧分压(PaO2)；取肺测定湿/干质量(W/D)比值、肺毛细血管通透性及组织病理学改变；酶联免疫吸附法(ELISA)检测血清肿瘤坏死因子(TNF-α)及白介素-6(IL-6)水平；逆转录-聚合酶链反应(RT-PCR)测定肺组织Slit2、Robo4mRNA表达；免疫组织化学法观察Slit2、Robo4蛋白表达情况。对照组每个时间点8只大鼠，ALI组每个时间点16只大鼠。结果：与对照组比较，ALI组致伤后各时间点PaO2均显著降低，肺W/D比值、肺毛细血管通透性、血清TNF-α及IL-6水平均显著升高（P＜0.05）；病理观察显示肺组织受损；ALI组Slit2表达下降（P＜0.05），Robo4表达差异无统计学意义(P0.05)。结论：正常大鼠肺组织可表达Slit2及Robo4，，ALI大鼠肺组织Slit2表达下降，这可能与ALI发病有关。
[Abstract]:Aim: to observe the changes of Slit2 and Robo4 in the lung of acute lung injury (ALI) rats caused by cecal ligation and perforation, and to explore the role of them in the pathogenesis and development of ALI. Methods Seventy-two male SD rats were randomly divided into two groups: control group (n = 24) and ALI group (n = 48). The model of ALI was established by cecal ligation and perforation in Ali group. Arterial blood oxygen partial pressure (PaO2), wet / dry mass ratio (W / D), pulmonary capillary permeability and histopathological changes were measured at 12: 24 hours after injury. Serum levels of tumor necrosis factor (TNF- 伪) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA), the expression of Slit2,Robo4mRNA in lung tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of Slit2,Robo4 protein was observed by immunohistochemistry. Control group (n = 8) and Ali group (n = 16). Results: compared with control group, PaO2 was significantly decreased, lung W / D ratio, pulmonary capillary permeability, serum TNF- 伪 and IL-6 levels were significantly increased in Ali group (P < 0. 05). There was no significant difference in Slit2 expression in ALI group (P < 0. 05). Conclusion: the expression of Slit2 and Slit2 in lung tissue of normal rats and Robo4,ALI rats can be decreased, which may be related to the pathogenesis of ALI.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563

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