被动吸烟增加幼鼠日后发展为哮喘的气道炎症

发布时间：2018-09-10 10:57

【摘要】：目的:在整体水平探索幼鼠被动吸烟对日后哮喘形成后气道炎症反应的影响。方法:幼鼠被动吸烟3周,进入成年后建立OVA致敏的BALB/c小鼠哮喘模型。40只BALB/c小鼠随机分为4组:生理盐水/空气组、生理盐水/被动吸烟组、OVA/空气组和OVA/被动吸烟组,每组10只。通过HE染色检测小鼠肺组织病理变化,细胞计数检测肺泡灌洗液中细胞(嗜酸性粒细胞、中性粒细胞、淋巴细胞、巨噬细胞)数目,ELISA方法检测肺泡灌洗液中细胞因子(IL-4、IL-5、IL-13)和血清中OVA-IgE、OVA-IgG1、OVA-IgG2a的表达情况,并检测各组小鼠的气道反应性。结果:幼年时期被动吸烟暴露可显著增加小鼠成年后OVA致敏哮喘模型的气道高反应性,血清OVA-IgE、OVA-IgG1、OVA-IgG2a表达,气道炎性细胞浸润和炎性细胞因子分泌(P0.05)。结论:幼年期被动吸烟可显著增加OVA致敏成年小鼠的气道炎症和气道高反应性,这可能为哮喘的防治提供新思路。
[Abstract]:Aim: to explore the effect of passive smoking on airway inflammation after asthma formation in young rats. Methods: after 3 weeks of passive smoking, 40 BALB/c mice were randomly divided into 4 groups: normal saline / air group, saline / passive smoking group and OVA/ passive smoking group. 10 rats in each group. The pathological changes of lung tissue were detected by HE staining, and the cells (eosinophilic granulocyte, neutrophil, lymphocyte) in alveolar lavage fluid were detected by cell count. The expression of cytokines (IL-4,IL-5,IL-13) in alveolar lavage fluid (IL-4,IL-5,IL-13) and OVA-IgE,OVA-IgG1,OVA-IgG2a in serum and airway reactivity in each group were detected by Elisa. Results: exposure to passive smoking during infancy significantly increased airway hyperresponsiveness, serum OVA-IgE,OVA-IgG1,OVA-IgG2a expression, airway inflammatory cell infiltration and inflammatory cytokine secretion in adult mice with OVA induced asthma (P0.05). Conclusion: passive smoking during infancy can significantly increase airway inflammation and airway hyperresponsiveness in OVA sensitized adult mice, which may provide a new idea for the prevention and treatment of asthma.
【作者单位】：吉林大学第一医院小儿呼吸一科;
【基金】：国家自然科学基金项目(81200013) 吉林大学白求恩B计划项目(2012202)资助
【分类号】：R562.25

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