哮喘及呼吸道合胞病毒感染小鼠的神经生长因子、白血病抑制因子的表达及地塞米松的影响

发布时间：2018-10-15 20:24

【摘要】：目的探讨神经生长因子(NGF)、白血病抑制因子(LIF)在支气管哮喘小鼠及呼吸道合胞病毒(RSV)感染小鼠的表达,两者是否存在相同的神经源性机制,寻找治疗哮喘的新靶点。方法100只健康Balb/c纯系小鼠随机分为5组:正常对照组,RSV组,哮喘组,哮喘合并RSV组,地塞米松组。HE染色观察肺组织病理变化,RT-PCR技术进行肺组织NGF mRNA及LIF mRNA的定量分析,免疫组织化学方法检测NGF蛋白及LIF蛋白。结果 RSV组镜下可见肺泡隔增宽,肺泡上皮肿胀,间质水肿。哮喘组及哮喘合并RSV组小鼠肺泡间隔增宽,支气管管腔变小,管壁增厚,小血管、细支气管及肺泡周围可见大量炎症性细胞,上述改变哮喘合并RSV组较哮喘组表现明显,而地塞米松组较RSV组表现减轻,正常对照组无炎症细胞浸润。各组均有NGF mRNA及LIF mRNA、NGF蛋白及LIF蛋白表达,哮喘合并RSV组表达最强,哮喘组及RSV组均较正常对照组表达增多,地塞米松组较RSV组表达减少。结论 RSV感染时肺组织NGF及LIF表达有所增加,合并哮喘时表达显著增加,地塞米松干预对NGF及LIF的表达均有一定的抑制作用。
[Abstract]:Objective to investigate the expression of nerve growth factor (NGF), leukemia inhibitory factor (LIF) in asthmatic mice and mice infected with respiratory syncytial virus (RSV). Methods 100 healthy Balb/c mice were randomly divided into 5 groups: normal control group, RSV group, asthma combined with RSV group, dexamethasone group. The pathological changes of lung tissue were observed by HE staining. The quantitative analysis of NGF mRNA and LIF mRNA in lung tissue was performed by RT-PCR technique. NGF protein and LIF protein were detected by immunohistochemistry. Results in RSV group, alveolar septum widened, alveolar epithelium swelling and interstitial edema were observed. The alveolar septum widens, the bronchial lumen become smaller, the wall thickens, and a large number of inflammatory cells are seen in the small blood vessels, bronchioles and around the alveoli in the asthmatic group and the asthmatic combined with RSV group. The above changes are more obvious in the asthmatic complicated with RSV group than in the asthmatic group. In the dexamethasone group, there was no inflammatory cell infiltration in the control group. The expression of NGF mRNA and LIF mRNA,NGF protein and LIF protein were observed in all groups. The expression of RSV was the strongest in asthmatic group and RSV group, but decreased in dexamethasone group compared with RSV group. Conclusion the expression of NGF and LIF in lung tissue was increased in RSV infection group and in asthmatic tissue. Dexamethasone intervention could inhibit the expression of NGF and LIF to some extent.
【作者单位】：徐州医学院;徐州医学院附属医院儿科;
【分类号】：R562.25

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