卡介苗干预对哮喘小鼠干细胞因子表达的影响

发布时间：2018-10-15 20:28

【摘要】：目的研究卡介苗(BCG)对哮喘小鼠干细胞因子(SCF)表达的影响。方法昆明小鼠随机分为哮喘组、BCG组和对照组,每组10只,以卵清蛋白(OVA)致敏激发建立哮喘模型。末次激发24 h后取肺组织及支气管肺泡灌洗液(BALF),计数嗜酸性粒细胞(EOS);酶联免疫吸附法检测BALF中SCF水平;免疫组织化学图像分析技术测定肺组织中SCF蛋白表达。结果哮喘组、BCG组和对照组肺组织的EOS计数分别为(10.67±1.94)个/HP、(6.40±1.55)个/HP和(0.37±0.33)个/HP,BALF中EOS计数分别为(7.58±1.30)×107/L、(3.78±1.15)×107/L和0,三组间差异有统计学意义(P均0.01)。哮喘组、BCG组和对照组的肺组织SCF蛋白免疫组化指数分别为(24 787.97±7 214.12)、(20 509.50±4 775.27)和(12 261.66±3 277.65),BALF中SCF水平分别为(280.25±14.20)pg/ml、(266.77±31.15)pg/ml和(223.59±15.61)pg/ml,其中哮喘组和BCG组均高于对照组,差异有统计学意义(P均0.05),BCG组与哮喘组差异无统计学意义(P0.05)。哮喘组肺部SCF表达与EOS计数呈正相关(P0.05)。结论 BCG干预能显著减轻哮喘小鼠的气道炎症,但不能抑制SCF的表达。
[Abstract]:Objective to study the effect of BCG (BCG) on the expression of stem cell factor (SCF) in asthmatic mice. Methods Kunming mice were randomly divided into asthma group, BCG group and control group with 10 mice in each group. Asthmatic model was established by sensitizing mice with ovalbumin (OVA). The (BALF), count of lung tissue and bronchoalveolar lavage fluid (BALF) was obtained 24 h after the last challenge. The level of SCF in BALF was detected by eosinophil (EOS); enzyme-linked immunosorbent assay (Elisa), and the expression of SCF protein in lung tissue was detected by immunohistochemical image analysis. Results the EOS counts of lung tissues in asthma group, BCG group and control group were (10.67 卤1.94) / HP, (6.40 卤1.55) / HP and (0.37 卤0.33) / HP,BALF, respectively (7.58 卤1.30) 脳 107 / L, (3.78 卤1.15) 脳 107 / L and 0, respectively. The immunohistochemical index of SCF protein in lung tissue of asthma group, BCG group and control group was (24 787.97 卤7 214.12), (20 509.50 卤4 775.27) and (12 261.66 卤3 277.65), BALF) respectively, and the SCF level in asthma group was (280.25 卤14.20) pg/ml, (266.77 卤31.15) pg/ml and (223.59 卤15.61) pg/ml, respectively. There was no significant difference between), BCG group and asthma group (P0.05). There was a positive correlation between SCF expression and EOS count in asthma group (P0.05). Conclusion BCG intervention can significantly reduce airway inflammation in asthmatic mice, but can not inhibit the expression of SCF.
【作者单位】：福建省厦门大学附属第一医院儿科;上海交通大学附属第六人民医院儿科;
【分类号】：R562.25

【相似文献】