PGD2调控TGF-β1在慢性阻塞性肺疾病气道重塑中的作用
发布时间：2018-10-20 18:45
【摘要】：目的探讨前列腺素D2(PGD2)对L-929小鼠肺成纤维细胞生物学特性的影响,利用其受体特异性抑制剂Laropiprant调控TGF-β1/Smads信号通路。方法按Laropiprant浓度梯度将细胞分为0.3μmoL组、1.0μmoL组、3.0μmoL组、10.0μmoL组、30.0μmoL,每组分别加入TGF-β2(2.5ng/mL)培养24h后使用相应浓度的Laropiprant刺激24h,并分别用PCR法和Western blot法检测细胞TGF-β1、Smad3以及Smad4的表达。分别采用不同浓度的Laropiprant用于细胞不同时间(12、24、48、72h),通过MTT法检测Laropiprant对细胞生长的抑制作用。结果随着Laropiprant的浓度增加,细胞TGF-β1、Smad3以及Smad4的mRNA和蛋白的表达呈下降趋势,与对照组比较差异有统计学意义(P0.05)。不同浓度的Laropiprant作用于细胞不同时间后,细胞抑制率随浓度增高和作用时间增长呈下降趋势。结论 L-929小鼠肺成纤维细胞PGD2-DP1的表达可能与TGF-β1/Smads的调节相关。Laropiprant作用于细胞后,细胞抑制率随浓度增高和作用时间增长呈下降趋势。
[Abstract]:Objective to investigate the effects of prostaglandin D 2 (PGD2) on the biological characteristics of lung fibroblasts in L-929 mice, and to regulate the TGF- 尾 1/Smads signaling pathway by Laropiprant, a receptor specific inhibitor of prostaglandin D 2 (PGD2). Methods the cells were divided into 0. 3 渭 moL group, 1. 0 渭 moL group, 3. 0 渭 moL group, 10. 0 渭 moL group and 30. 0 渭 moL, group according to the Laropiprant concentration gradient. The cells were incubated with TGF- 尾 2 (2.5ng/mL) for 24 h. The expression of TGF- 尾 1 Smad3 and Smad4 were detected by PCR and Western blot methods. The inhibitory effects of Laropiprant on cell growth were detected by MTT assay at different concentrations of Laropiprant for different time (12 ~ (24) O ~ (48) ~ (72) h). Results with the increase of Laropiprant concentration, the expression of mRNA and protein of TGF- 尾 1, Smad3 and Smad4 decreased significantly compared with the control group (P0.05). After different concentrations of Laropiprant were treated for different time, the inhibition rate of cells decreased with the increase of concentration and time of action. Conclusion the expression of PGD2-DP1 in lung fibroblasts of L-929 mice may be related to the regulation of TGF- 尾 1/Smads. After treated with Laropiprant, the inhibitory rate of cells decreased with the increase of concentration and time of action.
【作者单位】： 贵州省遵义市第一人民医院呼吸内科;
【基金】：遵义市2010年科学技术基金项目[遵市科合字(2010)27号]
【分类号】：R563.9
[Abstract]:Objective to investigate the effects of prostaglandin D 2 (PGD2) on the biological characteristics of lung fibroblasts in L-929 mice, and to regulate the TGF- 尾 1/Smads signaling pathway by Laropiprant, a receptor specific inhibitor of prostaglandin D 2 (PGD2). Methods the cells were divided into 0. 3 渭 moL group, 1. 0 渭 moL group, 3. 0 渭 moL group, 10. 0 渭 moL group and 30. 0 渭 moL, group according to the Laropiprant concentration gradient. The cells were incubated with TGF- 尾 2 (2.5ng/mL) for 24 h. The expression of TGF- 尾 1 Smad3 and Smad4 were detected by PCR and Western blot methods. The inhibitory effects of Laropiprant on cell growth were detected by MTT assay at different concentrations of Laropiprant for different time (12 ~ (24) O ~ (48) ~ (72) h). Results with the increase of Laropiprant concentration, the expression of mRNA and protein of TGF- 尾 1, Smad3 and Smad4 decreased significantly compared with the control group (P0.05). After different concentrations of Laropiprant were treated for different time, the inhibition rate of cells decreased with the increase of concentration and time of action. Conclusion the expression of PGD2-DP1 in lung fibroblasts of L-929 mice may be related to the regulation of TGF- 尾 1/Smads. After treated with Laropiprant, the inhibitory rate of cells decreased with the increase of concentration and time of action.
【作者单位】： 贵州省遵义市第一人民医院呼吸内科;
【基金】：遵义市2010年科学技术基金项目[遵市科合字(2010)27号]
【分类号】：R563.9
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