μ阿片受体激动剂DAMGO对创伤-内毒素二次打击所致大鼠急性肺损伤的影响

发布时间：2018-11-08 14:29

【摘要】：目的探讨μ阿片受体激动剂DAMGO对创伤-内毒素二次打击所致大鼠急性肺损伤的影响。方法 SD成年大鼠32只,随机分为正常对照组(N组)、空白对照组(B组)、DAMGO组(D组)和DAMGO+μ阿片受体拮抗剂CTOP组(DC组)。采用双侧股骨中段闭合性骨折6h后腹腔注射脂多糖(Lipopolysaccharides,LPS)5 mg/kg造模。N组只麻醉不造模,B组造模15min后腹腔注射等量生理盐水,D组注射DAMGO 200 μg/kg,DC组注射CTOP 600 μg/kg,5 min后注射DAMGO200 μg/kg。药物注射6h后行动脉血气分析,观察并比较肺组织病理改变,检测肺组织干湿重比、肿瘤坏死因子-α(TNF-α)、IL-6、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)活性。结果二次打击后大鼠肺功能降低,肺组织出现了明显炎症反应。与D组比较,B、DC组动脉血PaO2、pH值明显降低;肺组织Smith评分明显升高(P0.05);肺组织干湿重比明显降低(P0.05);肺组织MDA、TNF-α、IL-6含量明显升高(P0.05),SOD活性明显降低(P0.05)。结论μ阿片受体激动剂DAMGO对创伤-内毒素二次打击所致大鼠急性肺损伤有一定保护作用。
[Abstract]:Objective to investigate the effect of 渭 opioid receptor agonist (DAMGO) on acute lung injury induced by trauma and endotoxin in rats. Methods Thirty-two adult SD rats were randomly divided into normal control group (N group), blank control group (), DAMGO group D) and DAMGO 渭 opioid receptor antagonist CTOP group (DC group). The model was established by intraperitoneal injection of lipopolysaccharide (Lipopolysaccharides,LPS) for 5 mg/kg after 6 hours of bilateral closed fracture of femur. Group N was anesthetized only, group B was injected with normal saline at the same dose after 15min, group D was injected with DAMGO 200 渭 g / kg. In DC group, CTOP was injected with CTOP 600 渭 g / kg / kg for 5 min and then DAMGO200 渭 g / kg 路kg ~ (-1). Arterial blood gas analysis was performed 6 hours after drug injection. Pathological changes of lung tissue were observed and compared. Lung tissue dry-wet weight ratio, tumor necrosis factor- 伪 (TNF- 伪) and IL-6, were detected. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). Results the pulmonary function was decreased and the inflammatory reaction was observed in the lung tissue after the second blow. Compared with group D, the arterial blood PaO2,pH value was significantly decreased, the lung tissue Smith score was significantly increased (P0.05), the lung dry-wet weight ratio was significantly decreased (P0.05). The content of MDA,TNF- 伪 and IL-6 in lung tissue was significantly increased (P0.05), SOD activity was significantly decreased (P0.05). Conclusion 渭 opioid receptor agonist DAMGO can protect acute lung injury induced by trauma and endotoxin in rats.
【作者单位】：广东医学院;北京军区总医院附属八一脑科医院麻醉科;
【分类号】：R563.8

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