sPD-1在老年COPD患者外周血中的表达及意义

发布时间：2018-11-17 13:47

【摘要】：目的：研究可溶性程序性死亡因子-l(sPD-1)在老年慢性阻塞性肺疾病（COPD）患者及老年慢性阻塞性肺疾病急性加重期（AECOPD）患者外周血的表达。初步探讨其是否参与了COPD的发病机制。方法：选择老年COPD患者(31例)为研究对象。其中稳定期COPD患者(COPD组)10例，老年COPD急性加重期患者(AECOPD组)31例（包括COPD稳定期随访的10名患者急性加重期）。20例老年健康人为正常对照组。分别用ELISA试剂盒检测外周血中sPD-1的表达情况。统计结果采用SPSS13.0进行分析。结果：老年COPD及AECOPD患者、健康老年人血清中均存在适量sPD-1的表达。血清中sPD-1在老年COPD者含量为（51.11±9.90ng/ml）；而老年AECOPD患者为（51.70±9.33ng/ml），均低于健康生理条件下表达水平(92.53±12.00ng/ml)，两者与正常对照组相比均有显著性差异（P＜0.05）。对随访的10名老年COPD患者检测其稳定期和急性加重期血清中sPD-1含量进行比较，两者间差异无统计学意义（P＝0.70）。结论：本研究观察了sPD-1在健康老年人、老年患者COPD稳定期及急性加重期血清中的表达，结果显示与正常对照组相比，AECOPD组、COPD组外周血中sPD-1表达均明显降低(P0．05)。提示老年COPD患者体内存在sPD-1的表达下调，可能导致体内正负性刺激信号失去了平衡的相互作用，致使体内负性共刺激信号增多，使细胞免疫处于被抑制状态。导致慢性炎性反应。因此，，我们推测sPD-1应该有参与COPD的细胞免疫应答反应。然而，对随访COPD患者稳定期与急性加重期外周血sPD-1水平无统计学差异，可能与纳入研究数目不足有关，有待进一步研究。
[Abstract]:Aim: to investigate the expression of soluble programmed death factor-1 (sPD-1) in peripheral blood of elderly patients with chronic obstructive pulmonary disease (COPD) and aged patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). To explore whether it is involved in the pathogenesis of COPD. Methods: 31 elderly patients with COPD were selected. There were 10 patients with stable COPD (COPD group), 31 elderly patients with acute exacerbation of COPD (AECOPD group) (including 10 patients with acute exacerbation of COPD) and 20 healthy people as normal control group. The expression of sPD-1 in peripheral blood was detected by ELISA kit. The statistical results were analyzed by SPSS13.0. Results: in elderly patients with COPD and AECOPD, and in healthy elderly patients, there was a moderate expression of sPD-1 in serum. The content of sPD-1 in serum in elderly patients with COPD was (51.11 卤9.90ng/ml). The expression level of AECOPD in elderly patients was (51.70 卤9.33ng/ml), lower than that in healthy subjects (92.53 卤12.00ng/ml), and there was a significant difference between the two groups (P < 0. 05). The levels of serum sPD-1 in 10 elderly patients with COPD in stable and acute exacerbations were compared. There was no significant difference between the two groups (P0. 70). Conclusion: in this study, we observed the expression of sPD-1 in the serum of healthy elderly and elderly patients during the stable and acute exacerbation of COPD. The results showed that compared with the normal control group, the expression of sPD-1 in the AECOPD group was higher than that in the normal control group. The expression of sPD-1 in peripheral blood of COPD group was significantly decreased (P0.05). It is suggested that the down-regulation of sPD-1 expression in elderly patients with COPD may lead to the imbalance of positive and negative stimuli, increase the number of negative co-stimuli in vivo and inhibit cellular immunity. Causes chronic inflammatory reactions. Therefore, we speculate that sPD-1 should be involved in the cellular immune response to COPD. However, there was no significant difference in the level of peripheral blood sPD-1 between stable and acute exacerbations of COPD patients, which may be related to the insufficient number of inclusive studies, and need further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.9

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