地塞米松对哮喘小鼠肺组织中TWEAK及其受体Fn14表达的调节作用

发布时间：2018-12-09 12:35

【摘要】：目的:探讨地塞米松对哮喘小鼠模型肺组织中肿瘤坏死因子样弱凋亡诱导因子(TWEAK)及受体成纤维细胞生长因子诱导的早期反应蛋白14(Fn14)表达的调节作用。方法:采用卵清蛋白致敏方法建立经典哮喘模型。将36只雌性BALB/c小鼠,随机分为对照组、哮喘组、地塞米松组,每组12只。HE染色评估各组小鼠气道炎症程度;采用逆转录聚合酶链反应(RT-PCR)方法检测各组肺组织TWEAK、Fn14mRNA表达水平;采用免疫组化方法检测各组肺组织TWEAK、Fn14蛋白表达水平。结果:哮喘组小鼠肺组织TWEAK、Fn14 mRNA水平高于对照组(P0.01),地塞米松组TWEAK、Fn14 mRNA水平低于哮喘组(P0.01)。TWEAK、Fn14蛋白的表达水平哮喘组较对照组明显升高(P0.01),地塞米松组表达量较哮喘组明显降低(P0.01)。结论:地塞米松可抑制哮喘小鼠肺组织中TWEAK及Fn14表达,从而减少气道炎症反应。
[Abstract]:Aim: to investigate the effect of dexamethasone on the expression of tumor necrosis factor-like weak apoptosis-inducing factor (TWEAK) and receptor fibroblast growth factor-induced early reactive protein 14 (Fn14) in lung tissues of asthmatic mice. Methods: the classical asthma model was established by ovalbumin sensitizing method. Thirty-six female BALB/c mice were randomly divided into control group, asthma group and dexamethasone group (12 mice in each group). The degree of airway inflammation in each group was evaluated by HE staining. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of TWEAK,Fn14mRNA in lung tissues and immunohistochemical method was used to detect the expression of TWEAK,Fn14 protein in lung tissues. Results: the level of TWEAK,Fn14 mRNA in lung tissue of asthmatic group was higher than that of control group (P0.01), and the level of TWEAK,Fn14 mRNA in dexamethasone group was lower than that in asthmatic group (P0.01). The expression of TWEAK,Fn14 protein in asthmatic group was significantly higher than that in control group (P0.01). The expression of dexamethasone was significantly lower than that of asthma (P0.01). Conclusion: dexamethasone can inhibit the expression of TWEAK and Fn14 in the lung tissue of asthmatic mice and reduce airway inflammation.
【作者单位】：南方医科大学呼吸科;南方医科大学心内科;
【基金】：中华医学会慢性呼吸道疾病专项基金(08020460124)
【分类号】：R562.25

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