ω-3PUFAs、ω-6PUFAs和ω-9MUFAs对LPS诱导急性肺损伤大鼠血清IL-6及细胞凋亡的影响

发布时间：2018-12-09 19:18

【摘要】：目的比较ω-3PUFAs、ω-6PUFAs和ω-9MUFAs三种脂肪乳剂对脂多糖(lipid emulsions,LPS)诱导的急性肺损伤大鼠IL-6分泌的影响。方法 100只大鼠按随机数字分为Control组、LPS组、Intra组、Clino组和Omega组。分别采用生理盐水或不同种类脂肪乳剂处理7d;并均在取标本前8h时于气管内滴入生理盐水或LPS,建立ALI大鼠模型;观察各组大鼠肺组织的病理改变、凋亡指数,测定IL-6mRNA变化以及IL-6蛋白浓度。结果 1)各组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血;2)Clino组和Omega组血清中IL-6mRNA表达较Intra组、LPS组均明显降低;BALF中Clino组和Omega组IL-6蛋白水平明显低于Intra组、LPS组,差异具有统计学意义(P均0.005)。而Clino组和Omega组两组IL-6mRNA表达及其蛋白水平差异均无统计学意义(P均0.005)。3)Clino组和Omega组凋亡系数均低于LPS组和Intra组(P均0.005);Clino组和Omega组AI值差异无统计学意义(P0.005)。结论 Clinoleic和Omegaven可通过降低IL-6而减轻ALI大鼠炎症反应,并能减轻肺泡上皮细胞凋亡。
[Abstract]:Objective to compare the effects of 蠅 -3PUFAs, 蠅-6PUFAs and 蠅-9MUFAs on IL-6 secretion in rats with acute lung injury induced by lipopolysaccharide (lipid emulsions,LPS). Methods 100 rats were randomly divided into Control group, LPS group, Intra group, Clino group and Omega group. The rats were treated with normal saline or different kinds of fat emulsion for 7 days, and the ALI rat model was established by intratracheal instillation of normal saline or LPS, at 8 hours before the specimen was taken. The pathological changes, apoptosis index, changes of IL-6mRNA and the concentration of IL-6 protein were observed. Results (1) the expression of IL-6mRNA in the serum of Clino group and Omega group was significantly lower than that of Intra group and LPS group. The level of IL-6 protein in BALF group and Omega group was significantly lower than that in Intra group and LPS group (P 0.005). However, there was no significant difference in IL-6mRNA expression and protein level between Clino group and Omega group (P 0.005). The apoptotic coefficients of Clino group and Omega group were lower than those of LPS group and Intra group (P 0.005). There was no significant difference in AI between Clino group and Omega group (P 0.005). Conclusion Clinoleic and Omegaven can attenuate the inflammatory reaction and the apoptosis of alveolar epithelial cells in ALI rats by decreasing IL-6.
【作者单位】：新乡医学院第一附属医院新生儿科;新乡医学院人体解剖教研室;新乡医学院第三附属医院新生儿科;温州医科大学附属第一医院胃肠外科;
【基金】：浙江省医学重点扶植学科“临床营养学(2007-F03)”
【分类号】：R563

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