miR-21对ADAMTS-1的调节在肺纤维化体内外实验中的研究

发布时间：2018-12-10 06:27

【摘要】：目的:探讨mi R-21在肺纤维化模型中对ADAMTS-1表达水平的调节作用。方法:(1)建立博来霉素诱导的小鼠肺纤维化模型和构建体外肺纤维化模型,应用q RT-PCR检测mi R-21表达水平,Western blot法观察ADAMTS-1蛋白的表达。(2)通过体外转染mi R-21相似剂(mimics)及抑制剂(inhibitors)至NIH3T3细胞,Westem blot法检测ADAMTS-1蛋白的表达。结果:(1)在小鼠肺纤维化模型中,与空白对照组比较,博来霉素处理后的3个时间点,小鼠肺组织mi R-21表达明显上调,而ADAMTS-1蛋白表达明显下调(P0.01)。(2)在构建体外肺纤维化模型中,NIH3T3细胞经5μg/L的TGF-β1刺激后,ADAMTS-1蛋白表达下调,mi R-21表达量明显上调(P0.01)。(3)对NIH3T3细胞转染mi R-21相似物及抑制剂后实验结果显示,高表达mi R-21后,NIH3T3细胞ADAMTS-1蛋白表达下调,反之亦然。结论:(1)体内外实验证实,肺纤维过程中mi R-21表达上升,ADAMTS-1蛋白表达下降。(2)mi R-21可负调控细胞ADAMTS-1的表达水平。
[Abstract]:Aim: to investigate the regulatory effect of mi R-21 on the expression of ADAMTS-1 in pulmonary fibrosis model. Methods: (1) establish bleomycin induced pulmonary fibrosis model in mice and establish pulmonary fibrosis model in vitro. The expression of mi R-21 was detected by Q RT-PCR. The expression of ADAMTS-1 protein was detected by Western blot. (2) the expression of ADAMTS-1 protein was detected by transfection of mi R-21 analogue (mimics) and (inhibitors) to NIH3T3 cell, Westem blot in vitro. Results: (1) compared with the blank control group, the expression of mi R-21 in lung tissue of mice treated with bleomycin was upregulated significantly at three time points after treatment with bleomycin. The expression of ADAMTS-1 protein was down-regulated (P0.01). (2). In the model of pulmonary fibrosis in vitro, the expression of ADAMTS-1 protein was down-regulated in NIH3T3 cells stimulated by 5 渭 g / L TGF- 尾 1. The expression of mi R-21 was significantly up-regulated (P0.01). (3). After transfection of mi R-21 analogues and inhibitors into NIH3T3 cells, the results showed that the expression of ADAMTS-1 in NIH3T3 cells was down-regulated after high expression of mi R-21, and vice versa. Conclusion: (1) the expression of mi R-21 increased and the expression of ADAMTS-1 protein decreased in vitro and in vivo. (2) mi R-21 negatively regulated the expression of ADAMTS-1.
【作者单位】：南华大学护理学院;南华大学附属南华医院;
【基金】：湖南省自然科学基金资助项目(编号:14JJ7044) 湖南省教育厅资助项目(编号:14B157)
【分类号】：R563

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