支气管哮喘患者外周血中IL-33水平的检测及其在支气管哮喘发病机制中的作用

发布时间：2018-12-10 12:25

【摘要】：目的:检测支气管哮喘患者外周血中血清白细胞介素33(IL-33)的水平,探讨其与哮喘患者肺功能、免疫球蛋白E(IgE)的关系及其在支气管哮喘发病机制中的作用。方法:选择50例支气管哮喘患者作为实验组,均行肺功能及IgE水平检测,依据血清IgE水平将哮喘患者分为IgE升高组(n=25)和IgE正常组(n=25),另外选取20名健康对照者作为健康对照组,采用ELISA法检测受试者外周血中血清IL-33水平,比较健康者和哮喘患者急性发作期及临床控制期血清IL-33水平、第1秒用力呼气容积(FEV1)和呼气峰流速(PEF);分析IL-33水平与肺功能和IgE水平的相关性。结果:支气管哮喘急性发作期患者血清IL-33水平明显高于健康对照组(P0.05);IgE升高组患者血清IL-33高于IgE正常组(P0.05);临床控制期患者血清IL-33水平低于急性发作期者(P0.05),但高于健康对照组(P0.05)。哮喘急性发作期患者血清IL-33与患者FEV1和PEF均呈明显负相关关系(r=-0.413,P=0.003;r=-0.504,P0.001);哮喘临床控制期患者血清IL-33水平与患者FEV1和PEF亦呈负相关关系(r=-0.386,P=0.006;r=-0.324,P=0.022)。结论:IL-33作为一种炎性因子参与了哮喘的免疫-炎症反应机制,并可能加剧哮喘患者肺功能恶化。
[Abstract]:Aim: to detect the level of serum interleukin 33 (IL-33) in the peripheral blood of patients with bronchial asthma, and to investigate its relationship with lung function, immunoglobulin E (IgE) and its role in the pathogenesis of bronchial asthma. Methods: 50 patients with bronchial asthma were selected as experimental group. Lung function and IgE level were measured. According to the serum IgE level, asthmatic patients were divided into two groups: IgE elevated group (nt25) and IgE normal group (nnm25). In addition, 20 healthy controls were selected as the healthy control group. The serum IL-33 levels in peripheral blood were measured by ELISA method, and the serum IL-33 levels were compared between healthy subjects and asthmatic patients during acute attack and clinical control. Forced expiratory volume (FEV1) and peak expiratory flow rate (PEF);) To analyze the correlation between IL-33 level and lung function and IgE level. Results: the level of serum IL-33 in patients with acute attack of bronchial asthma was significantly higher than that in healthy controls (P0.05). The level of serum IL-33 in patients with elevated); IgE was significantly higher than that in patients with normal IgE (P0.05). The level of serum IL-33 in patients with clinical control was lower than that in patients with acute attack (P0.05), but higher than that in healthy controls (P0.05). There was a negative correlation between serum IL-33 and FEV1 and PEF in patients with acute attack of asthma (r = -0.413). There was also a negative correlation between serum IL-33 level and FEV1 and PEF in patients with asthma at clinical controlled stage (r-0.386P ~ (0.006) ~ (-0.324) P ~ + 0. 022). Conclusion: as an inflammatory factor, IL-33 is involved in the immune-inflammatory response mechanism of asthma, and may aggravate the deterioration of pulmonary function in asthmatic patients.
【作者单位】：吉林大学第一医院呼吸内科;
【基金】：吉林省科技厅科研基金资助课题(3D511Y833428) 吉林大学研究生创新基金项目资助课题(20121121)
【分类号】：R562.25

【参考文献】