煤工尘肺患者血清中血管内皮生长因子抗体的表达

发布时间：2018-12-13 05:30

【摘要】：目的检测煤工尘肺患者及接尘工人血清中血管内皮生长因子抗体(VEGF-Ab)的表达水平,探讨其在尘肺病发病过程中的意义。方法将对象分为4组:以230例煤工尘肺观察对象(已出现尘肺病变但尚未达到壹期尘肺)为观察对象组,以328例煤工尘肺患者为煤工尘肺组,以309例正在接尘的工人为接尘组(尚未出现尘肺病变),以393例医院健康体检人员为对照组,对所有研究对象进行问卷调查,并采集外周静脉血,分离收集血清,采用酶联免疫吸附试验(ELISA)法检测血清中VEGF-Ab的表达水平。结果煤工尘肺组和接尘组血清中VEGF-Ab表达水平均高于健康对照组和观察对象组(P0.05)。随着尘肺病期别的增高,煤工尘肺组患者血清中VEGF-Ab的表达水平虽有增加的趋势,但差异无统计学意义(P0.05)。观察对象组中20~岁组和40~岁组VEGF-Ab表达水平均高于60~岁组(P0.05);煤工尘肺组和健康对照组中20~岁组和40~岁组VEGF-Ab表达水平均低于60~岁组(P0.05)。煤工尘肺组中采煤组血清VEGF-Ab高于辅助工种组(P0.05),接尘组中掘进组血清VEGF-Ab高于采煤组(P0.05)。观察对象组和煤工尘肺组的壹期尘肺组中,工龄25年的患者血清中VEGF-Ab表达低于工龄≥25年者(P0.05)。结论血清中VEGF-Ab的表达水平可能与煤工尘肺的发生发展过程相关,随着年龄的增大、接尘工龄的增加VEGF-Ab的表达水平升高。
[Abstract]:Objective to detect the expression of vascular endothelial growth factor antibody (VEGF-Ab) in serum of coal worker pneumoconiosis patients and dust-exposed workers, and to explore its significance in the pathogenesis of pneumoconiosis. Methods the subjects were divided into four groups: 230 cases of coal workers' pneumoconiosis (who had appeared pneumoconiosis but did not reach stage 1 pneumoconiosis) and 328 cases of coal workers' pneumoconiosis (CWP). 309 workers who were exposed to dust were used as dust exposure group (without pneumoconiosis) and 393 healthy persons in hospital as control group. All the subjects were investigated by questionnaire, and peripheral venous blood was collected to collect serum. The expression of VEGF-Ab in serum was detected by enzyme linked immunosorbent assay (ELISA). Results the expression of VEGF-Ab in serum of coal worker pneumoconiosis group and dust-exposed group was higher than that of healthy control group and observation group (P0.05). With the increase of other pneumoconiosis stage, the expression of VEGF-Ab in the serum of coal worker pneumoconiosis patients had an increasing trend, but the difference was not statistically significant (P0.05). The expression of VEGF-Ab in 20 ~ year old group and 40 ~ year old group was higher than that in 60 ~ year old group (P0.05), and the VEGF-Ab expression level in 20 ~ year old group and 40 ~ year old group in coal worker pneumoconiosis group and healthy control group was lower than that in 60 ~ year old group (P0.05). The serum VEGF-Ab of coal mining group was higher than that of auxiliary work group (P0.05), and the serum VEGF-Ab of dust exposure group was higher than that of coal mining group (P0.05). The expression of VEGF-Ab in the first stage pneumoconiosis group of the target group and the coal worker pneumoconiosis group was lower than that in the patients with 25 years of service (P0.05). Conclusion the expression of VEGF-Ab in serum may be related to the occurrence and development of coal worker pneumoconiosis. With the age, the expression level of VEGF-Ab increases with the age of exposure to dust.
【作者单位】：郑州大学公共卫生学院劳动卫生与职业病学教研室;
【基金】：国家自然科学基金(No.81072278)资助
【分类号】：R135.2

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