建立监测血流动力学变化的兔急性肺血栓栓塞模型

发布时间：2019-01-23 16:24

【摘要】：目的应用猪尾导管建立能够监测血流动力学变化的兔急性肺血栓栓塞(PTE)模型。方法试验兔24只随机分为PTE组和对照组,每组12只,均建立右侧颈静脉和左侧颈动脉通路。PTE组经导管注入自体血栓3个,通过肺动脉造影评估PTE模型是否成功,监测肺动脉压力和血气分析等变化。结果造模成功率100%。PTE组肺动脉造影显示,右心室扩张,肺动脉增粗、充盈缺损、截断或完全缺失。造模后,PTE组较造模前和对照组肺动脉平均压明显增高[(16.40±1.82)mm Hg vs(9.60±2.07)mm Hg和(11.17±2.14)mm Hg,1 mm Hg=0.133kPa,P=0.01,P=0.002]。血气分析显示,造模后PTE组较造模前pH、PO2、标准碳酸氢盐、血氧饱和度以及动脉/肺泡氧分压等指标明显降低,肺泡动脉氧分压差明显增加。结论经颈静脉送入猪尾导管建立兔急性PTE模型成功率较高,重复性较好,且能够监测血流动力学变化,为PTE诊治的相关研究提供了实验基础。
[Abstract]:Objective to establish a rabbit model of acute pulmonary thromboembolism (PTE) by using pig tail catheter to monitor hemodynamic changes. Methods Twenty-four rabbits were randomly divided into PTE group and control group, 12 in each group. The right jugular vein and left carotid artery were established in each group. 3 autologous thrombus were injected through catheter in PTE group. The success of PTE model was evaluated by pulmonary arteriography. Pulmonary artery pressure and blood gas analysis were monitored. Results Pulmonary arteriography showed right ventricular dilatation, pulmonary artery thickening, filling defect, amputation or complete deletion in 100%.PTE group. After modeling, the mean pulmonary artery pressure in PTE group was significantly higher than that before model making and control group [(16.40 卤1.82) mm Hg vs (9.60 卤2.07) mm Hg and (11.17 卤2.14) mm Hg,1 mm Hg=0.133kPa,P=0.01,P=0.002]. Blood gas analysis showed that the pH,PO2, standard bicarbonate, blood oxygen saturation and arterial / alveolar partial pressure of oxygen in PTE group were significantly lower than those before model making, and the difference of oxygen pressure difference in alveolar artery was obviously increased. Conclusion the rabbit acute PTE model established through jugular vein into pig tail catheter has high success rate, good repeatability, and can monitor hemodynamic changes, which provides experimental basis for the study of diagnosis and treatment of PTE.
【作者单位】：天津医科大学心血管病临床学院
【基金】：天津市滨海新区卫生局医药卫生科技(2011BHKY006)
【分类号】：R563.5

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