呼吸道合胞病毒G蛋白基因序列分析

发布时间：2019-03-18 11:05

【摘要】：目的了解2010年四川地区呼吸道合胞病毒(HRSV)G蛋白基因特征和优势型别。方法 RTPCR方法扩增18例呼吸道感染HRSV阳性患儿样本HRSVG蛋白基因,测序并分析G蛋白基因特征,构建系统进化树鉴定基因型,分析选择压力。结果所测G蛋白基因第二个高变区序列7例A亚型和11例B亚型核苷酸基因距离分别为0.022±0.005和0.073±0.010,碱基转换率大于颠换率,A和B亚型分别主要是GA+AG和UC+CU转换。系统进化分析A亚型全部为GA2基因型,B亚型为BA基因型和GB2基因型;A亚型G蛋白基因长度都为298aa,B亚型为295aa、312aa和315aa3种长度。A、B亚型的选择压力为净化选择占主导,第二个高变区A亚型有9个正选择位点,B亚型有1个正选择位点。结论 2010年四川18例HRSV以GA2、GB2和BA基因型为主要型别,G蛋白基因长度变化、碱基替换、选择压力是HRSV逃避免疫反应的原因。
[Abstract]:Objective to understand the characteristics and dominant types of respiratory syncytial virus (HRSV) G protein gene in Sichuan in 2010. Methods RTPCR method was used to amplify the HRSVG gene of 18 HRSV positive children with respiratory tract infection. The G protein gene was sequenced and analyzed. Phylogenetic tree was constructed to identify the genotype and the selection pressure was analyzed. Results the nucleotide distances of 7 subtype A and 11 B subtypes of G protein gene were 0.022 卤0.005 and 0.073 卤0.010, respectively. The base conversion rate was higher than the transversion rate. Subtype A and B were mainly GA AG and UC CU transitions, respectively. Phylogenetic analysis showed that subtype A was GA2 genotype and subtype B was BA genotype and GB2 genotype. The length of G protein gene was 298aa in subtype A, 295aa in subtype B, 312aa and 315aa3 species length. The selection pressure of subtypes A and B was dominant in purification selection. There were 9 positive selection sites in subtype A and 1 positive selection site in subtype B in the second hypervariable region. Conclusion GA2,GB2 and BA genotypes were the main types of HRSV in Sichuan in 2010. G protein gene length change and base substitution were the main types of HRSV in 2010. Selection pressure was the reason why HRSV evaded immune response.
【作者单位】：四川大学华西公共卫生学院卫生检验与检疫学系;陕西省疾病预防控制中心;
【基金】：“艾滋病和病毒性肝炎等重大传染病防治科技”重大专项“传染病检测技术平台”项目 “云南省周边省市传染病病原谱流行规律研究”课题(No.2009ZX10004-212,No.2012ZX10004-212)资助
【分类号】：R563

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