骨髓间充质干细胞对染矽尘大鼠肺损伤修复作用研究

发布时间：2019-03-24 15:47

【摘要】：目的观察骨髓间充质干细胞(MSC)对矽尘所致大鼠肺损伤的修复作用。方法取7只无特定病原体级健康雄性SD大鼠培养MSC,并取30只同类SD大鼠随机分为对照组、染矽尘组和MSC输注组。对照组大鼠气管内注射1.0 ml生理氯化钠溶液,染矽尘组和MSC输注组均气管内注射1.0 ml质量浓度为40 g/L的矽尘混悬液,其后,MSC输注组大鼠鼠尾静脉注射0.5 ml细胞密度为5×109/L的MSC,其余2组鼠尾静脉注射0.5 ml生理氯化钠溶液。第28天处死大鼠,观察肺组织病理学改变情况,并检测肺脏器系数、肺灌洗液(BALF)细胞计数和血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)活力和尿素氮(BUN)、肌酐(Cr)水平。结果染矽尘组和MSC输注组大鼠的肺脏器系数均高于对照组[(0.528±0.044)%vs(0.435±0.027)%,(0.492±0.042)%vs(0.435±0.027)%,P0.05],MSC输注组肺脏器系数低于染矽尘组[(0.492±0.042)%vs(0.528±0.044)%,P0.05]。病理学检查显示:对照组无肺部炎症、矽结节和纤维化改变;染矽尘组大鼠肺部炎症最严重,并可见大量矽结节和胶原纤维沉积;与染矽尘组比较,MSC输注组肺部炎症减轻,矽结节数量减少,胶原纤维沉积面积较小。对照组大鼠BALF的总细胞数、巨噬细胞、中性粒细胞和淋巴细胞计数分别为(5.86±1.28)×106/L、(5.45±1.30)×106/L、(0.11±0.04)×106/L和(0.23±0.10)×106/L,染矽尘组的分别为(10.37±2.29)×106/L、(6.57±1.34)×106/L、(3.18±0.98)×106/L和(0.58±0.10)×106/L,MSC输注组的分别为(8.31±1.59)×106/L、(6.15±1.49)×106/L、(1.66±0.36)×106/L和(0.43±0.10)×106/L;染矽尘组和MSC输注组大鼠BALF的总细胞数、中性粒细胞和淋巴细胞计数均分别高于对照组(P0.05),MSC输注组上述3个指标均低于染矽尘组(P0.05)。3组血清ALT、AST、CK、LDH活力及BUN、Cr水平分别比较,差异均无统计学意义(P0.05)。结论骨髓MSC对矽尘导致的肺部炎症及纤维化有潜在修复作用。
[Abstract]:Objective to observe the repair effect of bone marrow mesenchymal stem cells (MSC) on lung injury induced by silica dust in rats. Methods 7 healthy male SD rats without specific pathogen grade were used to culture MSC, and 30 SD rats were randomly divided into three groups: control group, silica-exposed group and MSC infusion group. The rats in the control group were injected with 1.0 ml physiological sodium chloride solution, and the rats in the silicon dust group and the MSC infusion group were intratracheal injected with 40 g / L silica dust suspension at a mass concentration of 1.0 ml. The rats in the MSC infusion group were injected with 0.5 ml MSC, with a cell density of 5 脳 109 渭 L by tail vein. 0.5 ml physiological sodium chloride solution was injected into the tail vein of the other two groups. On the 28th day, the rats were killed to observe the pathological changes of lung tissue, and to detect the lung organ coefficient, (BALF) cell count in lung lavage fluid and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK),. Lactate dehydrogenase (LDH) activity and urea nitrogen (BUN), creatinine (Cr) level. Results the lung organ coefficient of rats exposed to silica dust and MSC infusion group was higher than that of control group [(0.528 卤0.044)% vs (0.435 卤0.027)%, (0.492 卤0.042)% vs (0.435 卤0.027)%, P0.05]. The lung organ coefficient of MSC infusion group was lower than that of silica-exposed group [(0.492 卤0.042)% vs (0.528 卤0.044)%, P0.05]. Pathological examination showed that there were no pulmonary inflammation, silicosis nodules and fibrosis in the control group, and the lung inflammation was the most serious in the silica-exposed rats, and a large number of silicosis nodules and collagen fibers were found in the rats exposed to silica dust. Compared with the group exposed to silica dust, the pulmonary inflammation, the number of silicosis nodules and the deposition area of collagen fibers in the MSC infusion group were less than those in the control group. The total cell count, macrophage, neutrophil and lymphocyte count of BALF in the control group were (5.86 卤1.28) 脳 10 ~ 6 渭 L, (5.45 卤1.30) 脳 10 ~ 6 渭 L, (0.11 卤0.04) 脳 10 ~ 6 渭 L and (0.23 卤0.10) 脳 10 ~ 6 mL, respectively. (10.37 卤2.29) 脳 10 ~ 6 mL, (6.57 卤1.34) 脳 10 ~ 6 mL, (3.18 卤0.98) 脳 10 ~ 6 渭 L and (0.58 卤0.10) 脳 10 ~ 6 mL in the silica-exposed group, and (8.31 卤1.59) 脳 10 ~ 6 mL in the MSC infusion group, respectively. (6.15 卤1.49) 脳 10 ~ 6 / L, (1.66 卤0.36) 脳 10 ~ 6 / L and (0.43 卤0.10) 脳 10 ~ 6 / L; The total cell number, neutrophil and lymphocyte counts of BALF in silica-exposed group and MSC-infused group were higher than those in control group (P0.05). The above-mentioned three indexes in), MSC infusion group were lower than those in silica-exposed group (P0.05). Serum ALT,AST,CK, in three groups was significantly higher than that in control group (P0.05). There was no significant difference in the activity of LDH and the level of BUN,Cr (P0.05). Conclusion Bone marrow MSC has potential repair effect on lung inflammation and fibrosis induced by silica dust.
【作者单位】：广东省职业病防治院广东省职业病防治重点实验室;贵阳医学院;
【基金】：国家自然科学基金(81302396) 国家临床重点专科建设项目(2011-09) 广东省职业病防治重点实验室(2012A061400007) 广东省医学科学研究基金资助项目(A2010016) 广东省职业病防治院专项课题(Z200907)
【分类号】：R135.2

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