吸烟对支气管黏膜组织PPAR-γ、IL-8和TNF-α表达的影响

发布时间：2019-05-15 17:18

【摘要】：目的探讨吸烟对支气管黏膜组织过氧化物酶增殖物激活受体γ(PPAR-γ)、IL-8和TNF-α表达的影响及其临床意义。方法选取慢性阻塞性肺疾病(COPD)吸烟者12例(COPD吸烟组)、肺功能正常吸烟者13例(肺功能正常吸烟组)、肺功能正常不吸烟者15例(肺功能正常不吸烟组),采用免疫组化Max Visionfa法检测三组支气管黏膜组织PPAR-γ、IL-8、TNF-α蛋白表达,分析吸烟者(COPD吸烟组及肺功能正常吸烟组)支气管黏膜组织IL-8、TNF-α、PPAR-γ表达与FEV1、FEV1/FVC的关系。结果与肺功能正常不吸烟组比较,COPD吸烟组、肺功能正常吸烟组支气管黏膜组织PPAR-γ蛋白表达均下降,IL-8、TNF-α蛋白表达均升高,组间比较P均0.01。吸烟者(COPD吸烟组与肺功能正常吸烟组)支气管黏膜组织IL-8、TNF-α表达均与PPAR-γ表达呈负相关(r分别为-0.463、-0.391,P均0.05),IL-8表达均与FEV1、FEV1/FVC呈负相关(r分别为-0.458、-0.465,P均0.01),TNF-α表达均与FEV1、FEV1/FVC呈负相关(r分别为-0.495、-0.476,P均0.01),PPAR-γ表达均与FEV1、FEV1/FVC呈正相关(r分别为0.592、0.425,P均0.01)。结论吸烟可导致COPD及肺功能正常者支气管黏膜组织IL-8、TNF-α表达升高,PPAR-γ表达降低,三者水平变化可能是烟雾导致肺功能下降的原因。
[Abstract]:Objective to investigate the effect of smoking on the expression of peroxisome proliferator-activated receptor gamma (PPAR- 纬), IL-8 and TNF- 伪 in bronchial mucosa and its clinical significance. Methods 12 (COPD) smokers with chronic obstructive pulmonary disease (COPD smoking group), 13 smokers with normal pulmonary function (smoking group with normal pulmonary function) and 15 patients with normal pulmonary function (non-smoking group with normal pulmonary function) were selected. The expression of PPAR- 纬 and IL-8,TNF- 伪 protein in bronchial mucosa of three groups was detected by immunohistochemical Max Visionfa method, and the expression of IL-8,TNF- 伪 in bronchial mucosa of smokers (COPD smoking group and normal pulmonary function smoking group) was analyzed. The relationship between the expression of PPAR- 纬 and FEV1,FEV1/FVC. Results compared with the non-smoking group with normal pulmonary function, the expression of PPAR- 纬 protein and IL-8,TNF- 伪 protein in bronchial mucosa of COPD smoking group and normal pulmonary function smoking group were decreased and the expression of IL-8,TNF- 伪 protein was higher than that of non-smoking group (P < 0.01). The expression of IL-8,TNF- 伪 in bronchial mucosa of smokers (COPD smoking group and normal lung function smoking group) was negatively correlated with the expression of PPAR- 纬 (r =-0.463, P < 0.391, P < 0.05). The expression of IL-8 was negatively correlated with FEV1,. The expression of TNF- 伪 was negatively correlated with FEV1,FEV1/FVC (r =-0.458, r = 0.465, P < 0.01), the expression of TNF- 伪 was negatively correlated with FEV1,FEV1/FVC (r =-0.495, r = 0.476, P < 0.01), and the expression of PPAR- 纬 was negatively correlated with FEV1, (r =-0.458, P < 0.01). FEV1/FVC was positively correlated (r = 0.592, 0.425, P < 0.01). Conclusion smoking can increase the expression of IL-8,TNF- 伪 and decrease the expression of PPAR- 纬 in bronchial mucosa of patients with COPD and normal pulmonary function. The changes of the three levels may be the cause of the decrease of pulmonary function caused by smoke.
【作者单位】：山东省交通医院;
【基金】：山东省自然科学基金资助项目(Y2001C01) 山东省医药卫生科技发展计划(HZ059)
【分类号】：R563.9

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