间充质干细胞在哮喘大鼠气道炎症及气道重塑中的作用

发布时间：2019-05-15 21:36

【摘要】：目的探讨间充质干细胞(MSCs)在哮喘大鼠慢性气道炎症及气道重塑中的作用。方法将18只SD雌性大鼠随机分为:正常对照组、哮喘4周组和哮喘8周组,每组6只。卵清蛋白(OVA)致敏后,雾化吸入OVA制作哮喘模型。哮喘模型成功后,测定气道压力;通过HE染色、Image-Pro Plus图像分析软件分析大鼠气道平滑肌的嗜酸性粒细胞(ESO)浸润情况,测定支气管管腔的内周长、管壁面积,支气管平滑肌面积、平滑肌细胞核数;采用密度梯度离心法分离外周血单个核细胞,通过流式细胞仪检测各组MSCs的含量,并分析其与哮喘气道炎症及气道重塑的关系。结果哮喘4周组、哮喘8周组大鼠的气道反应性、气道壁EOS计数、支气管管壁面积、支气管平滑肌面积、平滑肌细胞核数目、外周血中MSCs比例均较正常对照组显著增加(P0.01,P0.05);哮喘两组间上述指标差异均有统计学意义(P0.01);Pearson线性相关显示:各组大鼠外周血单个核细胞中MSCs的含量与气道反应性、EOS浸润数、支气管壁面积、支气管平滑肌厚度及支气管平滑肌细胞核数均呈正相关(P0.01)。结论 MSCs在哮喘状态下含量增加,可能参与了哮喘的慢性气道炎症及气道重塑。
[Abstract]:Objective to investigate the role of (MSCs) in chronic airway inflammation and airway remodeling in asthma rats. Methods 18 female SD rats were randomly divided into normal control group, asthma 4 week group and asthma 8 week group with 6 rats in each group. After ovalbumin (OVA) was sensitized, the asthma model was established by atomizing inhaled OVA. After the asthma model was successful, the airway pressure was measured. The (ESO) infiltration of eosinophils in rat airway smooth muscle was analyzed by HE staining and Image-Pro Plus image analysis software. The inner circumference, wall area, bronchial smooth muscle area and nucleus number of smooth muscle cells were measured. Peripheral blood mononuclear cells were isolated by density gradient centrifugation. The content of MSCs in each group was detected by flow cytometry and its relationship with airway inflammation and airway remodeling in asthma was analyzed. Results Airway responsiveness, airway wall EOS count, bronchial wall area, bronchial smooth muscle area, number of smooth muscle cells in asthma group and 8 weeks asthma group were studied. The proportion of MSCs in peripheral blood was significantly higher than that in the control group (P0.01, P0.05). There were significant differences in the above indexes between the two groups (P 0.01). Pearson linear correlation showed that the content of MSCs in peripheral blood mononuclear cells was positively correlated with airway responsiveness, EOS infiltration, bronchial wall area, bronchial smooth muscle thickness and bronchial smooth muscle cell nucleus (P01). Conclusion the increase of MSCs content in asthma may be involved in chronic airway inflammation and airway remodeling in asthma.
【作者单位】：桂林医学院附属医院呼吸内科;衡阳市第一人民医院呼吸内科;柳州市工人医院呼吸内科;
【基金】：国家自然科学基金(编号:81260009) 广西自然科学基金重点课题(编号:2012GXNSFDA053020)
【分类号】：R562.25

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