血浆TLR-4、MMP-9和Ⅳ-C表达变化与肺部感染患者病情的关系

发布时间：2019-06-10 12:51

【摘要】：目的探讨血浆白细胞表面Toll样受体4(TLR-4)、基质金属蛋白酶-9(MMP-9)和IV型胶原蛋白(Ⅳ-C)水平变化与肺部感染的关系。方法肺部感染患者41例,根据病情严重程度分为全身炎症反应综合征(SIRS)组28例和多器官功能障碍综合征(MODS)组13例,另选取知情同意的20例健康志愿者作为对照组。清晨采集三组研究对象的肘静脉血,采用流式细胞术测定血浆TLR-4,ELISA法定量测定血浆MMP-9,放射免疫法测定血浆Ⅳ-C。对血浆TL-4、MMP-9和Ⅳ-C水平与病情严重程度进行相关性分析。结果对照组、SIRS组和MODS组血浆TLR-4、MMP-9和Ⅳ-C水平均逐渐升高,组间两两比较,P均0.05。血浆TLR-4、MMP-9、Ⅳ-C水平与病情严重程度均呈正相关(r分别为0.745、0.912、0.945,P均0.01)。结论血浆TLR-4、MMP-9、Ⅳ-C水平与肺部感染患者病情程度呈正相关关系。
[Abstract]:Objective to investigate the relationship between the levels of Toll-like receptor 4 (TLR-4), matrix metalloprotease-9 (MMP-9) and type IV collagen (IV-C) in plasma leukocytes and pulmonary infection. Methods 41 patients with pulmonary infection were divided into systemic inflammatory response syndrome (SIRS) group (28 cases) and multiple organ dysfunction syndrome (MODS) group) group (13 cases) according to the severity of the disease. 20 healthy volunteers with informed consent were selected as control group. The cubital vein blood of the three groups was collected in the early morning, and the plasma MMP-9, was quantitatively determined by flow cytometry (TLR-4,ELISA). Plasma IV-C. The correlation between plasma TL-4,MMP-9 and IV-C levels and the severity of the disease was analyzed. Results the plasma levels of TLR-4,MMP-9 and IV-C in the control group, SIRS group and MODS group were gradually increased, and the levels of plasma TLR-4,MMP-9 and IV-C in the control group and MODS group were significantly higher than those in the control group (P < 0.05). The plasma TLR-4,MMP-9, IV-C level was positively correlated with the severity of the disease (r = 0.745, 0.912, 0.945, P < 0.01). Conclusion there is a positive correlation between plasma TLR-4,MMP-9, IV-C level and the severity of pulmonary infection.
【作者单位】：三峡大学人民医院;宜昌市第一人民医院;
【分类号】：R563

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