Th17细胞及其细胞因子白细胞介素-17在粉尘螨致敏哮喘小鼠中的变化
发布时间：2019-07-20 07:43
【摘要】：目的探讨Th17细胞及其细胞因子白细胞介素-17(IL-17)在粉尘螨致敏哮喘小鼠中的变化及其意义。方法 20只BALB/c小鼠随机均分为哮喘组和健康对照组。哮喘组小鼠于第0、7和14天每鼠腹腔注射200μl致敏液[含粉尘螨粗浸液提取物50μg,Al(OH)32 mg]致敏。末次免疫后1周采用粉尘螨粗浸液提取物连续进行滴鼻激发,每天1次,每次50μg,激发7次,健康对照组给予等体积的PBS[含Al(OH)32 mg]处理。末次激发后24 h内处死小鼠,取血清和肺泡灌洗液,无菌取脾脏。ELISA检测血清中Ig G1、Ig E和肺泡灌洗液中IL-17的含量;流式细胞仪检测脾脏中Th17细胞百分率。结果哮喘组小鼠血清中Ig G1和Ig E水平分别为(0.10±0.01)pg/ml和(1.15±0.10)pg/ml,高于健康对照组的(0.06±0.01)pg/ml和(0.04±0.01)pg/ml(P0.05);哮喘组小鼠肺泡灌洗液IL-17水平(85.13±2.36)pg/ml高于健康对照组(48.27±4.14)pg/ml(P0.01);哮喘组小鼠脾脏Th17细胞百分率(5.19±0.68)%高于健康对照组(0.95±0.19)%(P0.01),且脾脏Th17细胞百分率与肺泡灌洗液中IL-17水平呈正相关(r=0.851,P0.01)。结论粉尘螨致敏哮喘小鼠较健康小鼠肺泡灌洗液IL-17水平和脾脏Th17细胞数量均升高。
[Abstract]:Objective to investigate the changes and significance of Th17 cells and their cytokine IL-17 (IL-17) in asthma mice sensitized by Dermatophagoides farinae. Methods 20 BALB/c mice were randomly divided into asthma group and healthy control group. In the asthma group, 200 渭 l sensitized solution (50 渭 g, Al (OH) 32 mg) was injected intraperitoneally on the 0th, 7th and 14th day. One week after the last immunization, the crude extract of Dermatophagoides farinae was used for nasal stimulation once a day, 50 渭 g each time for 7 times. The healthy control group was treated with the same volume of PBS [containing Al (OH) 32 mg]. The mice were killed within 24 hours after the last challenge, serum and alveolar lavage fluid were taken, and spleen was taken aseptically. The contents of Ig G1, IgE and IL-17 in serum and alveolar lavage fluid were detected by Elisa, and the percentage of Th17 cells in spleen was detected by flow cytometry. Results the serum levels of Ig G 1 and Ig E in asthma group were (0.10 卤0.001) pg/ml and (1.15 卤0.10) pg/ml, respectively, which were higher than those in healthy control group (0.006 卤0.01pg/ml and 0.004 卤0.01pg/ml, P 0.05), and the levels of IL-17 in alveolar lavage fluid in asthma group (85.13 卤2.36) pg/ml were higher than those in healthy control group (48.27 卤4.14) pg/ml (P 0.01), and the levels of IL-17 in alveolar lavage fluid in asthma group were significantly higher than those in healthy control group (P < 0.01). The percentage of Th17 cells in spleen of asthma group (5.19 卤0.68)% was higher than that of healthy control group (0.95 卤0.19)% (P 0.01), and the percentage of Th17 cells in spleen was positively correlated with the level of IL-17 in alveolar lavage fluid (r 鈮，
本文编号：2516576
[Abstract]:Objective to investigate the changes and significance of Th17 cells and their cytokine IL-17 (IL-17) in asthma mice sensitized by Dermatophagoides farinae. Methods 20 BALB/c mice were randomly divided into asthma group and healthy control group. In the asthma group, 200 渭 l sensitized solution (50 渭 g, Al (OH) 32 mg) was injected intraperitoneally on the 0th, 7th and 14th day. One week after the last immunization, the crude extract of Dermatophagoides farinae was used for nasal stimulation once a day, 50 渭 g each time for 7 times. The healthy control group was treated with the same volume of PBS [containing Al (OH) 32 mg]. The mice were killed within 24 hours after the last challenge, serum and alveolar lavage fluid were taken, and spleen was taken aseptically. The contents of Ig G1, IgE and IL-17 in serum and alveolar lavage fluid were detected by Elisa, and the percentage of Th17 cells in spleen was detected by flow cytometry. Results the serum levels of Ig G 1 and Ig E in asthma group were (0.10 卤0.001) pg/ml and (1.15 卤0.10) pg/ml, respectively, which were higher than those in healthy control group (0.006 卤0.01pg/ml and 0.004 卤0.01pg/ml, P 0.05), and the levels of IL-17 in alveolar lavage fluid in asthma group (85.13 卤2.36) pg/ml were higher than those in healthy control group (48.27 卤4.14) pg/ml (P 0.01), and the levels of IL-17 in alveolar lavage fluid in asthma group were significantly higher than those in healthy control group (P < 0.01). The percentage of Th17 cells in spleen of asthma group (5.19 卤0.68)% was higher than that of healthy control group (0.95 卤0.19)% (P 0.01), and the percentage of Th17 cells in spleen was positively correlated with the level of IL-17 in alveolar lavage fluid (r 鈮，
本文编号：2516576
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