吡格列酮对哮喘小鼠肺组织GABAARα、MUC5AC表达的影响

发布时间：2019-07-21 06:25

【摘要】：目的:观察吡格列酮对哮喘小鼠肺组织GABAARα、MUC5AC表达的影响。方法:30只成年BALB/C小鼠随机分为3组,每组10只。哮喘组和吡格列酮干预组以卵白蛋白(OVA)致敏、激发制作哮喘小鼠模型,吡格列酮干预组于每次激发前30 min雾化吸入吡格列酮5×10-5mol/L,对照组以生理盐水代替OVA处理。末次激发后24 h处死小鼠,取肺组织行HE染色观察气道病理学表现,采用免疫组化SP法、RT-RCR法检测肺组织GABAARα、MUC5AC蛋白和mRNA的表达。结果:哮喘组小鼠气道黏膜下充血水肿,黏膜层增厚,支气管壁及血管壁周围有较多炎性细胞浸润;吡格列酮干预组上述表现较哮喘组减轻。哮喘组小鼠肺组织GABAARα、MUC5AC蛋白和mRNA的表达水平均较对照组高,吡格列酮干预组较哮喘组降低,但仍高于对照组(P均0.05)。结论:吡格列酮可能通过下调GABAARα的表达在哮喘气道黏液过度分泌中起治疗作用。
[Abstract]:Objective: to observe the effect of pioglitazone on the expression of GABAAR 伪 and MUC5AC in lung tissue of asthma mice. Methods: thirty adult BALB/C mice were randomly divided into 3 groups with 10 mice in each group. Asthma mice were sensitized with ovalbumin (OVA) in asthma group and pioglitazone intervention group. Pioglitazone intervention group inhaled pioglitazone 5 脳 10 ~ (- 5) mol 路L 30 min before each challenge, and control group was treated with saline instead of OVA. The mice were killed 24 hours after the last challenge. The pathological features of airway were observed by HE staining. The expression of GABAAR 伪, MUC5AC protein and mRNA in lung tissue was detected by SP method and RT-RCR method. Results: in the asthma group, the airway submucous hyperemia and edema, the thickening of the mucous membrane and the infiltration of inflammatory cells around the bronchial wall and vascular wall were observed, and the above manifestations in the pioglitazone intervention group were less than those in the asthma group. The expression of GABAAR 伪, MUC5AC protein and mRNA in lung tissue of asthma group was higher than that of control group, and that of pioglitazone intervention group was lower than that of asthma group, but it was still higher than that of control group. Conclusion: pioglitazone may play a therapeutic role in the hypersecretion of airway mucus in asthma by down-regulating the expression of GABAAR 伪.
【作者单位】：郑州大学第二附属医院呼吸内科;
【基金】：河南省科技厅重点科技攻关项目122102310236
【分类号】：R562.25

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