丹酚酸B体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞

发布时间：2017-12-27 05:13

本文关键词：丹酚酸B体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞　出处：《中华老年心脑血管病杂志》2013年12期 　论文类型：期刊论文

【摘要】：目的探讨丹酚酸B体外诱导大鼠骨髓间充质干细胞(BMSC)分化为心肌细胞样的可行性。方法取SD大鼠四肢骨骨髓,分离培养BMSC,第2代BMSC经丹酚酸B诱导分化为诱导组,未诱导为对照组。相差显微镜观察细胞形态;免疫组织化学法检测结蛋白、心肌肌钙蛋白I(cTnI)、连接蛋白43(Cx43)、p38丝裂素活化蛋白激酶(MAPK)的表达;透射电镜观察分化细胞的超微结构。结果原代细胞接种4h后贴壁生长,48h后细胞呈纺锤形和长梭形,传代细胞经丹酚酸B诱导1周后细胞呈多角形、梭形等形状,部分细胞有分支,诱导4周后诱导组细胞形态明显改变,细胞核位于中央,呈柱状的细胞紧密平行排列,有明显方向性。诱导组细胞中均有结蛋白、cTnI、Cx43及p38MAPK表达,阳性率分别为40.0%、32.3%、53.5%和64.8%,对照组细胞呈弱阳性或阴性表达。免疫荧光检测显示,结蛋白与cTnI呈共表达。结论丹酚酸B体外可诱导大鼠BMSC分化为心肌样细胞。
[Abstract]:Objective to investigate the feasibility of the differentiation of rat bone marrow mesenchymal stem cells (BMSC) into cardiomyocytes in vitro by salvianolic acid (B). Methods the bone marrow of SD rats was isolated and cultured for BMSC. The second generation of BMSC was induced by salvianolic acid B into the induction group, which was not induced as the control group. The cell morphology was observed by phase contrast microscope. The expression of desmin, cardiac troponin I (cTnI), connexin 43 (Cx43) and p38 mitogen activated protein kinase (MAPK) were detected by immunohistochemistry. The ultrastructure of differentiated cells was observed by transmission electron microscope. The primary cells inoculated with 4H adherent growth after 48h cells were spindle and the spindle, cells after induced by salvianolic acid B 1 weeks after the cells were polygonal or fusiform shape, some cells have branches, after 4 weeks of induction induction group cell morphology changed obviously, the nucleus is located in the central, in the column the shape of the cells tightly arranged in parallel, with obvious directivity. The expression of desmin, cTnI, Cx43 and p38MAPK in the induced group was 40%, 32.3%, 53.5% and 64.8%, respectively, and the cells in the control group were weakly positive or negative. The immunofluorescence assay showed that the protein was co expressed with cTnI. Conclusion salvianolic acid B can induce rat BMSC to differentiate into myocardium like cells in vitro.
【作者单位】：河北北方学院;河北北方学院国际交流中心;河北北方学院组织学与胚胎学教研室;
【基金】：河北省高等学校科学研究计划(ZH2012001) 河北北方学院创新人才培育基金(CXRC1315)
【分类号】：R329.2
【正文快照】： 骨髓间充质干细胞(BMSC)具有很强的自我复制和横向分化潜能,在特定条件下可向脂肪细胞、成骨细胞、肌细胞等多种细胞分化,且在体外易于分离培养和扩增,在细胞治疗中发挥重要作用。BMSC在一定诱导剂的作用下可以定向分化为心肌样细胞。丹酚酸B是中药丹参的有效成分之一,大量研

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