正弦交变电磁场对体外培养大鼠股骨骨组织的影响

发布时间：2017-12-28 00:10

  本文关键词：正弦交变电磁场对体外培养大鼠股骨骨组织的影响　出处：《生物医学工程学杂志》2013年03期 　论文类型：期刊论文

  更多相关文章： 股骨 正弦交变电磁场 骨代谢

【摘要】：研究50Hz、1.8mT正弦交变电磁场(SEMFs)对体外培养大鼠股骨组织的影响。体外分离培养大鼠股骨组织,随机分为两组(磁场处理组和对照组)。磁场处理组用1.8mT的SEMFs每天处理股骨组织1.5h。在SEMFs处理后的第0d(第一次处理1.5h后)、1、2、3、4和5d,采用Real-time RT-PCR检测培养股骨组织中骨形成相关基因表达量的变化;在SEMFs处理后的第3、6、9和12d测定培养股骨组织中碱性磷酸酶(ALP)活性变化;在SEMFs处理后的3、6、9和12d测定培养股骨中钙含量的变化。结果表明,SEMFs能使OPG和Collagen-1mRNA表达量保持在一个相对稳定的水平,在SEMFs处理后第1d和5d明显增加Runx-2 mRNA的表达水平。在SEMFs处理后的3d和9dALP活性明显增高(P0.01)。SEMFs处理培养股骨组织6、9和12d后,钙含量明显高于对照组(P0.01)。50Hz、1.8mT SEMFs能促进体外培养大鼠股骨组织中骨代谢相关基因的表达;增加钙含量以及ALP活性。因此,本实验从体外培养股骨骨组织水平提供了SEMFs促进骨代谢的实验依据。
[Abstract]:To study the effect of 50Hz and 1.8mT sinusoidal electromagnetic field (SEMFs) on the tissue of rat femur in vitro. The rat femur tissues were isolated and cultured in vitro, and were randomly divided into two groups (magnetic field treatment group and control group). The magnetic field treatment group treated 1.5h of the femur tissue with 1.8mT SEMFs every day. In the first 0d after SEMFs treatment (for the first time after 1.5h treatment), 1, 2, 3, 4 and 5D, using Real-time RT-PCR to detect the expression of genes related to culture the formation of femoral bone tissues; Determination of alkaline phosphatase in tissue culture of femur after treatment with SEMFs third, sixth, ninth and 12D (ALP) activity was measured; calcium content of femur cultured after SEMFs treatment in 3, 6, 9 and 12D. The results showed that SEMFs could maintain OPG and Collagen-1mRNA expression at a relatively stable level. After SEMFs treatment, 1D and 5D significantly increased the expression level of Runx-2 mRNA. The activity of 3D and 9dALP increased significantly after SEMFs treatment (P0.01). After SEMFs treatment of 6, 9 and 12D femur tissues, the content of calcium was significantly higher than that of the control group (P0.01). 50Hz, 1.8mT SEMFs can promote the expression of cultured rat bone bone metabolism related genes in vitro; increase the calcium content and the activity of ALP. Therefore, this experiment provides the experimental basis for SEMFs to promote bone metabolism from the level of bone tissue culture in vitro.
【作者单位】： 兰州军区兰州总医院骨科研究所;
【基金】：甘肃省科技重大专项基金资助项目(09ZNKDA025)
【分类号】：R363
【正文快照】： 引言大量实验研究表明低频率低强度电磁场影响体外培养成骨细胞的增殖与分化[1-2]。有实验研究发现50Hz、1.8mT正弦交变电磁场(sinusoidal elec-tromagnetic fields,SEMFs)能促进体外成骨细胞成熟分化[3],同时本实验组研究也发现50Hz、1.8mTSEMFs每天处理成骨细胞1.5h能促进

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