氧化应激预处理的适应性保护作用

发布时间：2017-12-28 16:37

本文关键词：氧化应激预处理的适应性保护作用　出处：《临床心血管病杂志》2013年12期 　论文类型：期刊论文

【摘要】：目的:探讨过氧化氢(H2O2)预处理对人脐带间充质干细胞(hUC-MSCs)损伤的适应性保护作用。方法:HUC-MSCs体外分离培养、扩增纯化、鉴定后绘制生长曲线;对数期hUC-MSCs用不同浓度H2O2作用1h及12h,检测细胞代谢率;对数期hUC-MSCs用10、20、50、100mmo1/L H2O2预处理1h,恢复0h、12h或24h,用700、800、900mmo1/L H2O2损伤12h,观察细胞形态,细胞代谢率及细胞计数。结果:HUC-MSCs分离培养、扩增纯化后鉴定符合其表面标志,且3~6d进入对数期;H2O2诱导hUC-MSCs损伤呈浓度及时间依赖性;10、20、50、100mmo1/L H2O2预处理1h恢复12h减弱700、800、900mmo1/L H2O2作用12h引起的hUC-MSCs损伤。结论:低浓度短时间H2O2预处理对高浓度长时间H2O2引起的hUC-MSCs损伤具有适应性保护作用,且需要适宜的预处理强度及时间间隔。
[Abstract]:Objective: To investigate the adaptive protective effect of hydrogen peroxide (H2O2) preconditioning on human umbilical cord mesenchymal stem cells (hUC-MSCs) injury. Methods: isolation, amplification and purification and identification of HUC-MSCs after drawing the growth curve in vitro; logarithmic phase hUC-MSCs with different concentrations of H2O2 1H and 12h, the detection rate of cell metabolism; 10, 20, 50, 100mmo1/L H2O2 1H with pretreatment of logarithmic phase hUC-MSCs, 0h, 12h or 24h recovery, with 700, 800, 900mmo1/L H2O2 damage 12h, observation of cell morphology, cell metabolism and cell count. Results: the isolation, purification and identification was consistent with its surface markers HUC-MSCs and 3~6d in the logarithmic phase; H2O2 induced hUC-MSCs damage in a concentration and time dependent manner; 10, 20, 50, 100mmo1/L H2O2 pretreatment 1H recovery 12h 700, 900mmo1/L 800, H2O2 decreased 12h induced hUC-MSCs damage. Conclusion: low concentration and short time H2O2 pretreatment has an adaptive protective effect on hUC-MSCs damage induced by high concentration and long time H2O2, and needs appropriate pretreatment intensity and time interval.
【作者单位】：河南省新乡医学院;河南省人民医院心内科;
【基金】：河南省2012年重大科技攻关计划项目(No:122101310500)
【分类号】：R363
【正文快照】： 人脐带间充质干细胞(hUC-MSCs)存在于脐带结缔组织中,可分化为多种类型细胞,取材方便,增殖快速且免疫原性低,可作为治疗以细胞损害为特征的疾病的理想种子细胞。但损伤区缺血缺氧造成的氧化应激状态损害hUC-MSCs活性,限制了其应用。研究发现,低浓度过氧化氢(H2O2)预处理对骨

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