UT-B基因敲除小鼠睾丸间质细胞睾酮分泌水平变化及其对性成熟的影响

发布时间：2018-01-03 06:06

本文关键词：UT-B基因敲除小鼠睾丸间质细胞睾酮分泌水平变化及其对性成熟的影响　出处：《吉林大学学报(医学版)》2013年03期 　论文类型：期刊论文

【摘要】：目的:利用尿素通道蛋白B(UT-B)基因敲除小鼠模型探讨UT-B基因对雄性小鼠睾丸间质Leydig细胞分泌睾酮能力的影响。方法:饲养、繁殖并鉴定UT-B基因敲除(UT-B-/-)小鼠;选取4周龄遗传背景相同的野生型(UT-B+/+)雄性小鼠和UT-B-/-雄性小鼠各5只,采用放射免疫法检测2种小鼠血清睾酮和黄体生成素(LH)水平;分离培养并鉴定Leydig细胞,测定培养液上清中睾酮的水平。结果:成功获得UT-B-/-雄性小鼠和UT-B+/+雄性小鼠;成功分离培养原代Leydig细胞并经3β-HSD染色方法鉴定纯度达80%以上;4周龄UTB-/-雄性小鼠血清睾酮水平[(7.29±1.27)×10-2μg.L-1]明显高于UT-B+/+雄性小鼠[(5.53±1.58)×10-2μg.L-1](P0.05),2种小鼠血清LH水平比较差异无统计学意义(P0.05);UTB-/-雄性小鼠Leydig细胞培养上清中睾酮水平[(17.300±1.179)nmol.L-1]明显高于UT-B+/+雄性小鼠[(12.300±0.916)nmol.L-1](P0.01)。结论:4周龄UT-B-/-雄性小鼠Leydig细胞分泌睾酮的能力明显高于UT-B+/+小鼠,这可能是UT-B-/-雄性小鼠比UT-B+/+小鼠生殖育龄提前的原因之一。
[Abstract]:Objective: using the urea channel protein B (UT-B) gene knockout mouse model to investigate the effect of UT-B gene on male mouse Leydig cells Leydig testosterone secretion ability. Methods: feeding, breeding and identification of UT-B gene knockout (UT-B-/-) mice; selection of wild type at the age of 4 weeks with the same genetic background (UT-B+/+) and male mice each 5 UT-B-/- male mice were detected by radioimmunoassay, 2 serum testosterone and luteinizing hormone (LH) level in mice; isolation and identification of Leydig cell culture supernatant, determination of testosterone levels. Results: the success of UT-B- / UT-B+/+ male mice and male mice; primary cultured Leydig cells by 3 beta -HSD staining reached a purity of more than 80% successful isolation; 4 week old male UTB-/- mice serum testosterone level [(7.29 + 1.27) * 10-2 g.L-1] male mice were significantly higher than that of UT-B+/+ [(5.53 + 1.58) * 10-2 g.L-1] (P0.05), 2 mice blood No statistically significant differences in the levels of serum LH (P0.05); UTB-/- male mice Leydig cell culture supernatant testosterone [(17.300 + 1.179) nmol.L-1] male mice was significantly higher than that of UT-B+/+ [(12.300 + 0.916) nmol.L-1] (P0.01). Conclusion: the ability of 4 week old male UT-B-/- mice Leydig cells secreting testosterone was significantly higher than that in UT-B+/+ mice. This is probably UT-B-/- in male mice than in UT-B+/+ mice. One of the reasons for early reproductive age

【作者单位】：吉林大学白求恩医学院病理生理学系;黑龙江省五常市人民医院普外科;吉林亚泰生物药业股份有限公司;北京大学医学部药理学系;
【基金】：国家自然科学基金青年基金资助课题(81000271)
【分类号】：R392.1
【正文快照】： 尿素通道蛋白(urea transporters,UTs)是一组具有高度选择通透尿素能力的跨膜蛋白[1],目前已克隆出的尿素通道蛋白包括2个家族,即尿素通道蛋白A(UT-A)家族和尿素道通蛋白B(UT-B)家族。UT-A有6个亚型,主要分布在肾脏;UT-B表达于肾脏、红细胞、睾丸和脑等多个组织和器官[2-3]。

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